Long-term impact of cadmium shows little damage in Scenedesmus
acutiformis cultures

J 2017

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA a Josef HEDBAVNY

Základní údaje

Originální název

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

Autoři

KOVACIK, Jozef (703 Slovensko), Petr BABULA (203 Česká republika, garant, domácí), Viera PETERKOVA (703 Slovensko) a Josef HEDBAVNY (203 Česká republika)

Vydání

Algal Research - Biomass Biofuels and Bioproducts, Amsterdam, Elsevier Science BV. 2017, 2211-9264

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.745

Kód RIV

RIV/00216224:14110/17:00097503

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.algal.2017.04.029

UT WoS

000406305900022

Klíčová slova anglicky

Antioxidants; Axenic culture; Bioaccumulation; Fluorescence microscopy; Heavy metals

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 3. 2018 16:44, Soňa Böhmová

Anotace

V originále

Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.

Citovat

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA a Josef HEDBAVNY. Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures. Algal Research - Biomass Biofuels and Bioproducts. Amsterdam: Elsevier Science BV., 2017, roč. 25, JUL 2017, s. 184-190. ISSN 2211-9264. Dostupné z: https://dx.doi.org/10.1016/j.algal.2017.04.029.

@article{1389080,
   author = {Kovacik, Jozef and Babula, Petr and Peterkova, Viera and Hedbavny, Josef},
   article_location = {Amsterdam},
   article_number = {JUL 2017},
   doi = {http://dx.doi.org/10.1016/j.algal.2017.04.029},
   keywords = {Antioxidants; Axenic culture; Bioaccumulation; Fluorescence microscopy; Heavy metals},
   language = {eng},
   issn = {2211-9264},
   journal = {Algal Research - Biomass Biofuels and Bioproducts},
   title = {Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures},
   volume = {25},
   year = {2017}
}

TY  - JOUR
ID  - 1389080
AU  - Kovacik, Jozef - Babula, Petr - Peterkova, Viera - Hedbavny, Josef
PY  - 2017
TI  - Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures
JF  - Algal Research - Biomass Biofuels and Bioproducts
VL  - 25
IS  - JUL 2017
SP  - 184-190
EP  - 184-190
PB  - Elsevier Science BV.
SN  - 22119264
KW  - Antioxidants
KW  - Axenic culture
KW  - Bioaccumulation
KW  - Fluorescence microscopy
KW  - Heavy metals
N2  - Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.
ER  -

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA a Josef HEDBAVNY. Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures. \textit{Algal Research - Biomass Biofuels and Bioproducts}. Amsterdam: Elsevier Science BV., 2017, roč.~25, JUL 2017, s.~184-190. ISSN~2211-9264. Dostupné z: https://dx.doi.org/10.1016/j.algal.2017.04.029.

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