| VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK a Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. Stem Cells. UNITED STATES: WILEY-BLACKWELL, 2017, roč. 35, č. 9, s. 2050-2059. ISSN 1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.2660. |
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@article{1389283,
author = {Váňová, Tereza and Konečná, Žaneta and Zboňáková, Zuzana and La Venuta, Giuseppe and Zoufalová, Karolína and Jelínková, Šárka and Vařecha, Miroslav and Rotrekl, Vladimír and Krejčí, Pavel and Nickel, Walter and Dvořák, Petr and Bosáková, Michaela},
article_location = {UNITED STATES},
article_number = {9},
doi = {http://dx.doi.org/10.1002/stem.2660},
keywords = {Tyrosine kinase expressed in hepatocellular carcinoma; Fibroblast growth factor; Fibroblast growth factor 2; Pluripotent stem cells; Embryonic stem cells; Neural differentiation; Cardiac differentiation},
language = {eng},
issn = {1066-5099},
journal = {Stem Cells},
title = {Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion},
volume = {35},
year = {2017}
}
TY - JOUR
ID - 1389283
AU - Váňová, Tereza - Konečná, Žaneta - Zboňáková, Zuzana - La Venuta, Giuseppe - Zoufalová, Karolína - Jelínková, Šárka - Vařecha, Miroslav - Rotrekl, Vladimír - Krejčí, Pavel - Nickel, Walter - Dvořák, Petr - Bosáková, Michaela
PY - 2017
TI - Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion
JF - Stem Cells
VL - 35
IS - 9
SP - 2050-2059
EP - 2050-2059
PB - WILEY-BLACKWELL
SN - 10665099
KW - Tyrosine kinase expressed in hepatocellular carcinoma
KW - Fibroblast growth factor
KW - Fibroblast growth factor 2
KW - Pluripotent stem cells
KW - Embryonic stem cells
KW - Neural differentiation
KW - Cardiac differentiation
N2 - Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor (FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma (TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation.
ER -
VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK a Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. \textit{Stem Cells}. UNITED STATES: WILEY-BLACKWELL, 2017, roč.~35, č.~9, s.~2050-2059. ISSN~1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.2660.
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