Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC,
Controls Pluripotency and Early Cell Fate Decisions of Human
Pluripotent Stem Cells via Regulation of Fibroblast Growth
Factor-2 Secretion

J 2017

Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ et. al.

Základní údaje

Originální název

Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion

Autoři

VÁŇOVÁ, Tereza (203 Česká republika, domácí), Žaneta KONEČNÁ (203 Česká republika, domácí), Zuzana ZBOŇÁKOVÁ (703 Slovensko, domácí), Giuseppe LA VENUTA (276 Německo), Karolína ZOUFALOVÁ (203 Česká republika, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí), Miroslav VAŘECHA (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí), Pavel KREJČÍ (203 Česká republika, domácí), Walter NICKEL (276 Německo), Petr DVOŘÁK (203 Česká republika, garant, domácí) a Michaela BOSÁKOVÁ (203 Česká republika, domácí)

Vydání

Stem Cells, UNITED STATES, WILEY-BLACKWELL, 2017, 1066-5099

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.587

Kód RIV

RIV/00216224:14110/17:00094930

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/stem.2660

UT WoS

000408334300004

Klíčová slova anglicky

Tyrosine kinase expressed in hepatocellular carcinoma; Fibroblast growth factor; Fibroblast growth factor 2; Pluripotent stem cells; Embryonic stem cells; Neural differentiation; Cardiac differentiation

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 16:55, Soňa Böhmová

Anotace

V originále

Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor (FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma (TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation.

Návaznosti

GA15-23033S, projekt VaV

Název: Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk

Investor: Grantová agentura ČR, Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-33232A, projekt VaV

Název: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2

Citovat

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK a Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. Stem Cells. UNITED STATES: WILEY-BLACKWELL, 2017, roč. 35, č. 9, s. 2050-2059. ISSN 1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.2660.

@article{1389283,
   author = {Váňová, Tereza and Konečná, Žaneta and Zboňáková, Zuzana and La Venuta, Giuseppe and Zoufalová, Karolína and Jelínková, Šárka and Vařecha, Miroslav and Rotrekl, Vladimír and Krejčí, Pavel and Nickel, Walter and Dvořák, Petr and Bosáková, Michaela},
   article_location = {UNITED STATES},
   article_number = {9},
   doi = {http://dx.doi.org/10.1002/stem.2660},
   keywords = {Tyrosine kinase expressed in hepatocellular carcinoma; Fibroblast growth factor; Fibroblast growth factor 2; Pluripotent stem cells; Embryonic stem cells; Neural differentiation; Cardiac differentiation},
   language = {eng},
   issn = {1066-5099},
   journal = {Stem Cells},
   title = {Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion},
   volume = {35},
   year = {2017}
}

TY  - JOUR
ID  - 1389283
AU  - Váňová, Tereza - Konečná, Žaneta - Zboňáková, Zuzana - La Venuta, Giuseppe - Zoufalová, Karolína - Jelínková, Šárka - Vařecha, Miroslav - Rotrekl, Vladimír - Krejčí, Pavel - Nickel, Walter - Dvořák, Petr - Bosáková, Michaela
PY  - 2017
TI  - Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion
JF  - Stem Cells
VL  - 35
IS  - 9
SP  - 2050-2059
EP  - 2050-2059
PB  - WILEY-BLACKWELL
SN  - 10665099
KW  - Tyrosine kinase expressed in hepatocellular carcinoma
KW  - Fibroblast growth factor
KW  - Fibroblast growth factor 2
KW  - Pluripotent stem cells
KW  - Embryonic stem cells
KW  - Neural differentiation
KW  - Cardiac differentiation
N2  - Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor (FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma (TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation.
ER  -

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK a Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. \textit{Stem Cells}. UNITED STATES: WILEY-BLACKWELL, 2017, roč.~35, č.~9, s.~2050-2059. ISSN~1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.2660.

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