Carboxyl-anhydride and amine plasma coating of PCL nanofibers
to improve their bioactivity

J 2017

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK et. al.

Basic information

Original name

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Authors

MANAKHOV, Anton (643 Russian Federation, belonging to the institution), Eva DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution), Jiřina MEDALOVÁ (203 Czech Republic, belonging to the institution), Petra ČERNOCHOVÁ (203 Czech Republic, belonging to the institution), Adam OBRUSNÍK (203 Czech Republic, belonging to the institution), Miroslav MICHLÍČEK (203 Czech Republic, belonging to the institution), Dmitry V. SHTANSKY (643 Russian Federation) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

MATERIALS & DESIGN, OXFORD, ELSEVIER SCI LTD, 2017, 0264-1275

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.525

RIV identification code

RIV/00216224:14740/17:00097544

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.matdes.2017.06.057

UT WoS

000408688300028

Keywords in English

PCL nanofibers; Plasma polymerization; Cell viability; Amine surfaces; Carboxyl-anhydride layers

Abstract

V originále

The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.

Links

LD15150, research and development project

Name: Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Dmitry V. SHTANSKY and Lenka ZAJÍČKOVÁ. Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity. MATERIALS & DESIGN. OXFORD: ELSEVIER SCI LTD, 2017, vol. 132, October, p. 257-265. ISSN 0264-1275. Available from: https://dx.doi.org/10.1016/j.matdes.2017.06.057.

@article{1389322,
   author = {Manakhov, Anton and Dvořáková, Eva and Medalová, Jiřina and Černochová, Petra and Obrusník, Adam and Michlíček, Miroslav and Shtansky, Dmitry V. and Zajíčková, Lenka},
   article_location = {OXFORD},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.matdes.2017.06.057},
   keywords = {PCL nanofibers; Plasma polymerization; Cell viability; Amine surfaces; Carboxyl-anhydride layers},
   language = {eng},
   issn = {0264-1275},
   journal = {MATERIALS & DESIGN},
   title = {Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity},
   url = {http://www.sciencedirect.com/science/article/pii/S0264127517306469},
   volume = {132},
   year = {2017}
}

TY  - JOUR
ID  - 1389322
AU  - Manakhov, Anton - Dvořáková, Eva - Medalová, Jiřina - Černochová, Petra - Obrusník, Adam - Michlíček, Miroslav - Shtansky, Dmitry V. - Zajíčková, Lenka
PY  - 2017
TI  - Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity
JF  - MATERIALS & DESIGN
VL  - 132
IS  - October
SP  - 257-265
EP  - 257-265
PB  - ELSEVIER SCI LTD
SN  - 02641275
KW  - PCL nanofibers
KW  - Plasma polymerization
KW  - Cell viability
KW  - Amine surfaces
KW  - Carboxyl-anhydride layers
UR  - http://www.sciencedirect.com/science/article/pii/S0264127517306469
L2  - http://www.sciencedirect.com/science/article/pii/S0264127517306469
N2  - The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.
ER  -

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Dmitry V. SHTANSKY and Lenka ZAJÍČKOVÁ. Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity. \textit{MATERIALS \&{} DESIGN}. OXFORD: ELSEVIER SCI LTD, 2017, vol.~132, October, p.~257-265. ISSN~0264-1275. Available from: https://dx.doi.org/10.1016/j.matdes.2017.06.057.

Detailed Information on Publication Record