Carboxyl-anhydride and amine plasma coating of PCL nanofibers
to improve their bioactivity

J 2017

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK et. al.

Základní údaje

Originální název

Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Autoři

MANAKHOV, Anton (643 Rusko, domácí), Eva DVOŘÁKOVÁ (203 Česká republika, domácí), Jiřina MEDALOVÁ (203 Česká republika, domácí), Petra ČERNOCHOVÁ (203 Česká republika, domácí), Adam OBRUSNÍK (203 Česká republika, domácí), Miroslav MICHLÍČEK (203 Česká republika, domácí), Dmitry V. SHTANSKY (643 Rusko) a Lenka ZAJÍČKOVÁ (203 Česká republika, garant, domácí)

Vydání

MATERIALS & DESIGN, OXFORD, ELSEVIER SCI LTD, 2017, 0264-1275

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.525

Kód RIV

RIV/00216224:14740/17:00097544

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.matdes.2017.06.057

UT WoS

000408688300028

Klíčová slova anglicky

PCL nanofibers; Plasma polymerization; Cell viability; Amine surfaces; Carboxyl-anhydride layers

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 21:08, doc. Mgr. Lenka Zajíčková, Ph.D.

Anotace

V originále

The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.

Návaznosti

LD15150, projekt VaV

Název: Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Elektrostaticky zvlákněná biodegradabilní nanovlákna pokrytá plazmově připravenými aminovými vrstvami pro využití v biomedicíně

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Dmitry V. SHTANSKY a Lenka ZAJÍČKOVÁ. Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity. MATERIALS & DESIGN. OXFORD: ELSEVIER SCI LTD, 2017, roč. 132, October, s. 257-265. ISSN 0264-1275. Dostupné z: https://dx.doi.org/10.1016/j.matdes.2017.06.057.

@article{1389322,
   author = {Manakhov, Anton and Dvořáková, Eva and Medalová, Jiřina and Černochová, Petra and Obrusník, Adam and Michlíček, Miroslav and Shtansky, Dmitry V. and Zajíčková, Lenka},
   article_location = {OXFORD},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.matdes.2017.06.057},
   keywords = {PCL nanofibers; Plasma polymerization; Cell viability; Amine surfaces; Carboxyl-anhydride layers},
   language = {eng},
   issn = {0264-1275},
   journal = {MATERIALS & DESIGN},
   title = {Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity},
   url = {http://www.sciencedirect.com/science/article/pii/S0264127517306469},
   volume = {132},
   year = {2017}
}

TY  - JOUR
ID  - 1389322
AU  - Manakhov, Anton - Dvořáková, Eva - Medalová, Jiřina - Černochová, Petra - Obrusník, Adam - Michlíček, Miroslav - Shtansky, Dmitry V. - Zajíčková, Lenka
PY  - 2017
TI  - Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity
JF  - MATERIALS & DESIGN
VL  - 132
IS  - October
SP  - 257-265
EP  - 257-265
PB  - ELSEVIER SCI LTD
SN  - 02641275
KW  - PCL nanofibers
KW  - Plasma polymerization
KW  - Cell viability
KW  - Amine surfaces
KW  - Carboxyl-anhydride layers
UR  - http://www.sciencedirect.com/science/article/pii/S0264127517306469
L2  - http://www.sciencedirect.com/science/article/pii/S0264127517306469
N2  - The plasmamodification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groupswas achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion. (C) 2017 Elsevier Ltd. All rights reserved.
ER  -

MANAKHOV, Anton, Eva DVOŘÁKOVÁ, Jiřina MEDALOVÁ, Petra ČERNOCHOVÁ, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Dmitry V. SHTANSKY a Lenka ZAJÍČKOVÁ. Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity. \textit{MATERIALS \&{} DESIGN}. OXFORD: ELSEVIER SCI LTD, 2017, roč.~132, October, s.~257-265. ISSN~0264-1275. Dostupné z: https://dx.doi.org/10.1016/j.matdes.2017.06.057.

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