Inhibition of proteolysis by the hematophagous Eudiplozoon
nipponicum

a 2017

Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS et. al.

Základní údaje

Originální název

Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum

Autoři

ILGOVÁ, Jana (703 Slovensko, domácí), Lucie JEDLIČKOVÁ (203 Česká republika), Hana DVOŘÁKOVÁ (203 Česká republika), Libor MIKEŠ (203 Česká republika), Michal BENOVICS (703 Slovensko, domácí), Pavel ROUDNICKÝ (203 Česká republika, domácí), Jiří VOREL (203 Česká republika, domácí), Libor VOJTEK (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí), Jiří SALÁT (203 Česká republika, domácí), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)

Vydání

8th International Symposium on Monogenea, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

ECIP - European Centre of IchthyoParasitology

Kód RIV

RIV/00216224:14310/17:00094966

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8666-1

Klíčová slova anglicky

stefin; parasite; eudiplozoon; interaction host-parasite; inhibition;

Změněno: 24. 9. 2017 17:39, Mgr. Michal Benovics, Ph.D.

Anotace

V originále

Eudiplozoon nipponicum (Monogenea: Diplozoidae) is an obligatory hematophagous parasite of the common carp (Cyprinus carpio). The blood degradation by this species involves a cascade of cysteine and aspartic proteases hypothetically regulated by protease inhibitors (e.g. cystatins and stefins). These inhibitory molecules are also known to have impact on immunomodulation of the host and the repair of its tissue damaged by the parasite. Our study aims to reveal the biological function of the stefin of E. nipponicum which was detected in the transcriptome and excretory-secretory products of adult individuals. We prepared recombinant form of E. nipponicum stefin (rEnStef) in E. coli BL21 bacterial strain using pET19b expression plasmid vector. By adoption of fluorometric assay we observed efficient inhibition of cysteine peptidases (cathepsins L and B from E. nipponicum and mouse cathepsin L) via its conserved papain-binding domain. Surprisingly legumain (asparaginyl endopeptidase) inhibition was detected probably due to legumain-binding domain, untypical for stefins. rEnStef blocked proteolytic degradation of hemoglobin mediated by cysteine peptidases in the excretory-secretory products, soluble protein extracts from E. nipponicum and by recombinant cathepsins L3 and B of E. nipponicum, which manifests its role in blood digestion. rEnStef any effect on the activation of complement in carp’s plasma or oxidative burst in full blood studied using luminol-enhanced chemiluminescence. Significant downregulation of selected cytokines (IL-1beta, IL-8, TNF-alfa, IL-6 and IL-10) by LPS stimulated porcine alveolar macrophages and monocyte derived macrophages caused by rEnStef might indicate possible role of E. nipponicum stefin in immunomodulation of the host.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS, Pavel ROUDNICKÝ, Jiří VOREL, Libor VOJTEK, Pavel HYRŠL, Jiří SALÁT, Milan GELNAR a Martin KAŠNÝ. Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8666-1.

@proceedings{1390168,
   author = {Ilgová, Jana and Jedličková, Lucie and Dvořáková, Hana and Mikeš, Libor and Benovics, Michal and Roudnický, Pavel and Vorel, Jiří and Vojtek, Libor and Hyršl, Pavel and Salát, Jiří and Gelnar, Milan and Kašný, Martin},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {stefin; parasite; eudiplozoon; interaction host-parasite; inhibition;},
   language = {eng},
   isbn = {978-80-210-8666-1},
   title = {Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum},
   url = {http://ecip.cz/news/4},
   year = {2017}
}

TY  - CONF
ID  - 1390168
AU  - Ilgová, Jana - Jedličková, Lucie - Dvořáková, Hana - Mikeš, Libor - Benovics, Michal - Roudnický, Pavel - Vorel, Jiří - Vojtek, Libor - Hyršl, Pavel - Salát, Jiří - Gelnar, Milan - Kašný, Martin
PY  - 2017
TI  - Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum
SN  - 9788021086661
KW  - stefin
KW  - parasite
KW  - eudiplozoon
KW  - interaction host-parasite
KW  - inhibition;
UR  - http://ecip.cz/news/4
L2  - http://ecip.cz/news/4
N2  - Eudiplozoon nipponicum (Monogenea: Diplozoidae) is an obligatory hematophagous parasite of the common carp (Cyprinus carpio). The blood degradation by this species involves a cascade of cysteine and aspartic proteases hypothetically regulated by protease inhibitors (e.g. cystatins and stefins). These inhibitory molecules are also known to have impact on immunomodulation of the host and the repair of its tissue damaged by the parasite. Our study aims to reveal the biological function of the stefin of E. nipponicum which was detected in the transcriptome and excretory-secretory products of adult individuals. We prepared recombinant form of E. nipponicum stefin (rEnStef) in E. coli BL21 bacterial strain using pET19b expression plasmid vector. By adoption of fluorometric assay we observed efficient inhibition of cysteine peptidases (cathepsins L and B from E. nipponicum and mouse cathepsin L) via its conserved papain-binding domain. Surprisingly legumain (asparaginyl endopeptidase) inhibition was detected probably due to legumain-binding domain, untypical for stefins. rEnStef blocked proteolytic degradation of hemoglobin mediated by cysteine peptidases in the excretory-secretory products, soluble protein extracts from E. nipponicum and by recombinant cathepsins L3 and B of E. nipponicum, which manifests its role in blood digestion. rEnStef any effect on the activation of complement in carp’s plasma or oxidative burst in full blood studied using luminol-enhanced chemiluminescence. Significant downregulation of selected cytokines (IL-1beta, IL-8, TNF-alfa, IL-6 and IL-10) by LPS stimulated porcine alveolar macrophages and monocyte derived macrophages caused by rEnStef might indicate possible role of E. nipponicum stefin in immunomodulation of the host.
ER  -

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS, Pavel ROUDNICKÝ, Jiří VOREL, Libor VOJTEK, Pavel HYRŠL, Jiří SALÁT, Milan GELNAR a Martin KAŠNÝ. Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.

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