Changes of Cerebrospinal Fluid Peptides due to Tauopathy

J 2017

Changes of Cerebrospinal Fluid Peptides due to Tauopathy

MAJEROVA, Petra, Peter BARATH, Alena MICHALICOVA, Stanislav KATINA, Michal NOVAK et. al.

Základní údaje

Originální název

Changes of Cerebrospinal Fluid Peptides due to Tauopathy

Autoři

MAJEROVA, Petra (703 Slovensko), Peter BARATH (703 Slovensko), Alena MICHALICOVA (703 Slovensko), Stanislav KATINA (703 Slovensko, garant, domácí), Michal NOVAK (703 Slovensko) a Andrej KOVAC (703 Slovensko)

Vydání

Journal of Alzheimer’s Disease, Amsterdam, IOS Press, 2017, 1387-2877

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10103 Statistics and probability

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.476

Kód RIV

RIV/00216224:14310/17:00095001

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3233/JAD-170110

UT WoS

000402994400018

Klíčová slova anglicky

Cerebrospinal fluid; LC-MALDI MS; peptidomics; rat model; tauopathy

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2018 09:47, Ing. Nicole Zrilić

Anotace

V originále

Alzheimer’s disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes. Over the last decade, there has been an intensive search for novel biochemical markers for clinical diagnosis of AD and other tauopathies. In the present study, we used transgenic rat model for tauopathy expressing human truncated tau protein (aa 151–391/4R) to analyze the cerebrospinal fluid (CSF) peptidome using liquid chromatography – matrix assisted laser desorption/ionization mass spectrometry (LC-MALDI TOF/TOF). From 345 peptides, we identified a total of 175 proteins. Among them, 17 proteins were significantly altered in the CSF of transgenic rats. The following proteins were elevated in the CSF of transgenic rats when compared to the control animals: neurofilament light and medium chain, apolipoprotein E, gamma-synuclein, chromogranin A, reticulon-4, secretogranin-2, calsyntein-1 and -3, endothelin-3, neuroendocrine protein B72A, alpha-1-macroglobulin, and augurin. Interestingly most of the identified proteins were previously linked to AD and other tauopathies, indicating the significance of transgenic animals in biomarker validation.

Návaznosti

GA15-06991S, projekt VaV

Název: Analýza funkcionálních dat a související témata

Investor: Grantová agentura ČR, Analýza funkcionálních dat a související témata

Citovat

MAJEROVA, Petra, Peter BARATH, Alena MICHALICOVA, Stanislav KATINA, Michal NOVAK a Andrej KOVAC. Changes of Cerebrospinal Fluid Peptides due to Tauopathy. Journal of Alzheimer’s Disease. Amsterdam: IOS Press, 2017, roč. 58, č. 2, s. 507-519. ISSN 1387-2877. Dostupné z: https://dx.doi.org/10.3233/JAD-170110.

@article{1390822,
   author = {Majerova, Petra and Barath, Peter and Michalicova, Alena and Katina, Stanislav and Novak, Michal and Kovac, Andrej},
   article_location = {Amsterdam},
   article_number = {2},
   doi = {http://dx.doi.org/10.3233/JAD-170110},
   keywords = {Cerebrospinal fluid; LC-MALDI MS; peptidomics; rat model; tauopathy},
   language = {eng},
   issn = {1387-2877},
   journal = {Journal of Alzheimer’s Disease},
   title = {Changes of Cerebrospinal Fluid Peptides due to Tauopathy},
   url = {http://content.iospress.com/articles/journal-of-alzheimers-disease/jad170110},
   volume = {58},
   year = {2017}
}

TY  - JOUR
ID  - 1390822
AU  - Majerova, Petra - Barath, Peter - Michalicova, Alena - Katina, Stanislav - Novak, Michal - Kovac, Andrej
PY  - 2017
TI  - Changes of Cerebrospinal Fluid Peptides due to Tauopathy
JF  - Journal of Alzheimer’s Disease
VL  - 58
IS  - 2
SP  - 507-519
EP  - 507-519
PB  - IOS Press
SN  - 13872877
KW  - Cerebrospinal fluid
KW  - LC-MALDI MS
KW  - peptidomics
KW  - rat model
KW  - tauopathy
UR  - http://content.iospress.com/articles/journal-of-alzheimers-disease/jad170110
L2  - http://content.iospress.com/articles/journal-of-alzheimers-disease/jad170110
N2  - Alzheimer’s disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes. Over the last decade, there has been an intensive search for novel biochemical markers for clinical diagnosis of AD and other tauopathies. In the present study, we used transgenic rat model for tauopathy expressing human truncated tau protein (aa 151–391/4R) to analyze the cerebrospinal fluid (CSF) peptidome using liquid chromatography – matrix assisted laser desorption/ionization mass spectrometry (LC-MALDI TOF/TOF). From 345 peptides, we identified a total of 175 proteins. Among them, 17 proteins were significantly altered in the CSF of transgenic rats. The following proteins were elevated in the CSF of transgenic rats when compared to the control animals: neurofilament light and medium chain, apolipoprotein E, gamma-synuclein, chromogranin A, reticulon-4, secretogranin-2, calsyntein-1 and -3, endothelin-3, neuroendocrine protein B72A, alpha-1-macroglobulin, and augurin. Interestingly most of the identified proteins were previously linked to AD and other tauopathies, indicating the significance of transgenic animals in biomarker validation.
ER  -

MAJEROVA, Petra, Peter BARATH, Alena MICHALICOVA, Stanislav KATINA, Michal NOVAK a Andrej KOVAC. Changes of Cerebrospinal Fluid Peptides due to Tauopathy. \textit{Journal of Alzheimer’s Disease}. Amsterdam: IOS Press, 2017, roč.~58, č.~2, s.~507-519. ISSN~1387-2877. Dostupné z: https://dx.doi.org/10.3233/JAD-170110.

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