TLR-9 expression in dorsal root ganglia induced by Paclitaxel
treatment

a 2017

TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment

LEVIN, Shahaf, Kateřina KUBÍČKOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ, Marek JOUKAL et. al.

Základní údaje

Originální název

TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment

Autoři

LEVIN, Shahaf, Kateřina KUBÍČKOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL

Vydání

Morphology 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

978-80-263-1315-1

Změněno: 27. 9. 2017 12:35, MUDr. Shahaf Levin

Anotace

V originále

Chemotherapeutic agent Paclitaxel causes peripheral neuropathy based on alteration of calcium channels and defragmentation of mitochondria in peripheral nerve axons. These changes are related to the release of damage associated molecular patterns (DAMPs) which are associated with the expression of Toll-like receptor 9 (TLR-9). The aim of our study was to assess the changes of TLR-9 in the dorsal root ganglia (DRGs), related to Paclitaxel application. Wistar rats (n=18, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received vehiculum (alcohol and cremophor; 1:1). The animals were left to survive for 1, 3 and 7 days from the last application. Experimental and control rats were sacrificed together with naïve rats and perfused transcardially by Zamboni´s fixative. Longitudinal cryostat sections through the lumbar and cervical DRGs were cut and immunostained for TLR-9. Satellite glial cells (SGCs) were visualized using GFAP antibody. The intensity of TLR-9 immunofluorescence in the DRG was measured and statistically analyzed. The presence of TLR-9 immunofluorescence was found in the cytoplasm of both small-sized neurons and of satellite glial cells surrounding large-sized neurons, in both naïve and control animals. However, the application of Paclitaxel caused a visible presence of TLR-9 in medium-sized neurons as well. Statistically significant increase in TLR-9 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to control and naïve animals. The intensity of TLR-9 immunofluorescence increased with time of survival after the last application. Our results clearly indicate that DRGs react to Paclitaxel application with an increase of TLR-9 expression. These changes might be associated with inflammatory reaction in chemotherapy induced peripheral neuropathy.

Návaznosti

MUNI/A/0973/2016, interní kód MU

Název: Spoje a prostupnost hemato-likvorové bariéry po podání Paclitaxelu

Investor: Masarykova univerzita, Spoje a prostupnost hemato-likvorové bariéry po podání Paclitaxelu, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

LEVIN, Shahaf, Kateřina KUBÍČKOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment. In Morphology 2017. 2017. ISBN 978-80-263-1315-1.

@proceedings{1390852,
   author = {Levin, Shahaf and Kubíčková, Kateřina and Klusáková, Ilona and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2017},
   language = {eng},
   isbn = {978-80-263-1315-1},
   title = {TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment},
   year = {2017}
}

TY  - CONF
ID  - 1390852
AU  - Levin, Shahaf - Kubíčková, Kateřina - Klusáková, Ilona - Dubový, Petr - Joukal, Marek
PY  - 2017
TI  - TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment
SN  - 9788026313151
N2  - Chemotherapeutic agent Paclitaxel causes peripheral neuropathy based on alteration of calcium channels and defragmentation of mitochondria in peripheral nerve axons. These changes are related to the release of damage associated molecular patterns (DAMPs) which are associated with the expression of Toll-like receptor 9 (TLR-9). The aim of our study was to assess the changes of TLR-9 in the dorsal root ganglia (DRGs), related to Paclitaxel application. Wistar rats (n=18, males) were used in our experiments. Intraperitoneal injections of Paclitaxel in 4 doses with a cumulative dose of 8 mg/kg were performed on experimental rats, while control animals received vehiculum (alcohol and cremophor; 1:1). The animals were left to survive for 1, 3 and 7 days from the last application. Experimental and control rats were sacrificed together with naïve rats and perfused transcardially by Zamboni´s fixative. Longitudinal cryostat sections through the lumbar and cervical DRGs were cut and immunostained for TLR-9. Satellite glial cells (SGCs) were visualized using GFAP antibody. The intensity of TLR-9 immunofluorescence in the DRG was measured and statistically analyzed. The presence of TLR-9 immunofluorescence was found in the cytoplasm of both small-sized neurons and of satellite glial cells surrounding large-sized neurons, in both naïve and control animals. However, the application of Paclitaxel caused a visible presence of TLR-9 in medium-sized neurons as well. Statistically significant increase in TLR-9 immunofluorescence was found in the DRGs of Paclitaxel treated animals in comparison to control and naïve animals. The intensity of TLR-9 immunofluorescence increased with time of survival after the last application. Our results clearly indicate that DRGs react to Paclitaxel application with an increase of TLR-9 expression. These changes might be associated with inflammatory reaction in chemotherapy induced peripheral neuropathy.
ER  -

LEVIN, Shahaf, Kateřina KUBÍČKOVÁ, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. TLR-9 expression in dorsal root ganglia induced by Paclitaxel treatment. In \textit{Morphology 2017}. 2017. ISBN~978-80-263-1315-1.

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