Urinary intermediates of tryptophan as indicators of the gut
microbial metabolism

J 2017

Urinary intermediates of tryptophan as indicators of the gut microbial metabolism

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI et. al.

Základní údaje

Originální název

Urinary intermediates of tryptophan as indicators of the gut microbial metabolism

Autoři

PAVLOVÁ, Tereza (203 Česká republika, domácí), Veronika VIDOVÁ (203 Česká republika, domácí), Julie BIENERTOVÁ VAŠKŮ (203 Česká republika, domácí), Petr JANKŮ (203 Česká republika, domácí), Martina ALMÁŠI (203 Česká republika, domácí), Jana KLÁNOVÁ (203 Česká republika, domácí) a Zdeněk SPÁČIL (203 Česká republika, garant, domácí)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier Science, 2017, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.123

Kód RIV

RIV/00216224:14310/17:00095009

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2017.08.022

UT WoS

000410304700008

Klíčová slova anglicky

Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 4. 2018 17:51, Ing. Nicole Zrilić

Anotace

V originále

While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.

Návaznosti

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU

Název: RECETOX RI - OP VVV (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI - OP VVV, PO 1 Posilování kapacit pro kvalitní výzkum

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

GJ17-24592Y, projekt VaV

Název: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Grantová agentura ČR, Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

Citovat

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ a Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. Analytica Chimica Acta. Amsterdam: Elsevier Science, 2017, roč. 987, September, s. 72-80. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2017.08.022.

@article{1391002,
   author = {Pavlová, Tereza and Vidová, Veronika and Bienertová Vašků, Julie and Janků, Petr and Almáši, Martina and Klánová, Jana and Spáčil, Zdeněk},
   article_location = {Amsterdam},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.aca.2017.08.022},
   keywords = {Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Urinary intermediates of tryptophan as indicators of the gut microbial metabolism},
   url = {https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub},
   volume = {987},
   year = {2017}
}

TY  - JOUR
ID  - 1391002
AU  - Pavlová, Tereza - Vidová, Veronika - Bienertová Vašků, Julie - Janků, Petr - Almáši, Martina - Klánová, Jana - Spáčil, Zdeněk
PY  - 2017
TI  - Urinary intermediates of tryptophan as indicators of the gut microbial metabolism
JF  - Analytica Chimica Acta
VL  - 987
IS  - September
SP  - 72-80
EP  - 72-80
PB  - Elsevier Science
SN  - 00032670
KW  - Tryptophan metabolism
KW  - Gut microbiome
KW  - Urinary metabolome
KW  - Methyl indole-3-acetate
KW  - Methyl indol-3-propionate
KW  - N-acetyltryptophan
UR  - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
N2  - While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.
ER  -

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ a Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science, 2017, roč.~987, September, s.~72-80. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2017.08.022.

Podrobný výpis o publikaci