Polymer Pencil Graphite Electrode in the Study of
Electrochemical Processes of Guanine Analogues

D 2017

Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues

TRNKOVÁ, Libuše, Mehdi RAVANDEH a Jan HRBÁČ

Základní údaje

Originální název

Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues

Autoři

TRNKOVÁ, Libuše, Mehdi RAVANDEH a Jan HRBÁČ

Vydání

1. vyd. Providence, 68th Annual Meeting of the International Society of Electrochemistry, 1 s. 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10403 Physical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

guanin; polymerní pentelková elektroda; cyklická voltametrie; elektronová mikroskopie; Ramanova spektroskopie

Klíčová slova anglicky

guanine; polymer pencil graphite electrode; cyclic volatmmetry; electron microscopy; Raman spectroscopy

Štítky

Změněno: 17. 5. 2018 15:02, Ing. Nicole Zrilić

Anotace

V originále

Understanding the oxidation processes of purines and their derivatives is primary for the improvement of electrochemical methods of genetic and food materials analysis. However, it is generally known that guanine is one of the four main nucleobases found in the nucleic acids DNA and RNA, but it is less known that G, G-based purines, including the nucleoside and the nucleotide monophosphate play an important role in diet-related diuseases, helath of cardiovascular nad digestive systems and in neurodegenerative diseases. Electrochemical techniques in connection with carbon electrodes are powerful methods for analysis of G and its derivatives. Our previous research revelaed that a polymer pencil graphite electrode can serve as a sensitive and disposable sensor for purines, including G and its derivatives. In this work, the electrochemical oxidation processes of G, guanosine and guanosine monophosphate and their deoxy-derivatives were investigated. The dependence of oxidation signals on experimental conditions such as scan rate, electrode surface pretreatment, addition of Cu(II) ions, adsorption, pH and the presence of structural increments (ribofuranosyl-, deoxyribofuranosyl-, and phosphate group) alolowed us to evaluate:(i) kinetics and mechanism of G and GMP oxidation (electron and proton numbers, coefficients of electro-transfer, the role of electrode surface), (ii) the effect of adsorption and (iii) the effect of oxo, deoxy, and phosphate groups on oxidation responses. We can conclude that polymer pencil lead is a prospective electrode material with good stability and reproducibility not only for analysis of G and its analogues but also for whole purine group.

Návaznosti

LD15058, projekt VaV

Název: Příprava substrátů pro povrchově zesílenou Ramanovu spektroskopii pomocí elektrochemických, elektroforetických a jiskrový výboj využívajících technik. (Akronym: ELE-SERS)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Příprava substrátů pro povrchově zesílenou Ramanovu spektroskopii pomocí elektrochemických, elektroforetických a jiskrový výboj využívajících technik.

MUNI/A/1237/2016, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

TRNKOVÁ, Libuše, Mehdi RAVANDEH a Jan HRBÁČ. Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues. Online. In 68th Annual Meeting of the International Society of Electrochemistry. 1. vyd. Providence, 2017, 1 s.

@inproceedings{1391527,
   author = {Trnková, Libuše and Ravandeh, Mehdi and Hrbáč, Jan},
   address = {Providence},
   booktitle = {68th Annual Meeting of the International Society of Electrochemistry},
   edition = {1},
   keywords = {guanine; polymer pencil graphite electrode; cyclic volatmmetry; electron microscopy; Raman spectroscopy},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Providence},
   note = {Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+},
   title = {Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues},
   year = {2017}
}

TY  - JOUR
ID  - 1391527
AU  - Trnková, Libuše - Ravandeh, Mehdi - Hrbáč, Jan
PY  - 2017
TI  - Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues
CY  - Providence
N1  - Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+
KW  - guanine
KW  - polymer pencil graphite electrode
KW  - cyclic volatmmetry
KW  - electron microscopy
KW  - Raman spectroscopy
N2  - Understanding the oxidation processes of purines and their derivatives is primary for the improvement of electrochemical methods of genetic and food materials analysis. However, it is generally known that guanine is one of the four main nucleobases found in the nucleic acids DNA and RNA, but it is less known that G, G-based purines, including the nucleoside and the nucleotide monophosphate play an important role in diet-related diuseases, helath of cardiovascular nad digestive systems and in neurodegenerative diseases. Electrochemical techniques in connection with carbon electrodes are powerful methods for analysis of G and its derivatives. Our previous research revelaed that a polymer pencil graphite electrode can serve as a sensitive and disposable sensor for purines, including G and its derivatives. In this work, the electrochemical oxidation processes of G, guanosine and guanosine monophosphate and their deoxy-derivatives were investigated. The dependence of oxidation signals on experimental conditions such as scan rate, electrode surface pretreatment, addition of Cu(II) ions, adsorption, pH and the presence of structural increments (ribofuranosyl-, deoxyribofuranosyl-, and phosphate group) alolowed us to evaluate:(i) kinetics and mechanism of G and GMP oxidation (electron and proton numbers, coefficients of electro-transfer, the role of electrode surface), (ii) the effect of adsorption and (iii) the effect of oxo, deoxy, and phosphate groups on oxidation responses. We can conclude that polymer pencil lead is a prospective electrode material with good stability and reproducibility not only for analysis of G and its analogues but also for whole purine group.
ER  -

TRNKOVÁ, Libuše, Mehdi RAVANDEH a Jan HRBÁČ. Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues. Online. In \textit{68th Annual Meeting of the International Society of Electrochemistry}. 1. vyd. Providence, 2017, 1 s.

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