2017
Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection
HYRŠL, Pavel, Emad IBRAHIM, Pavel DOBEŠ, Martin KUNC, Dalibor KODRÍK et. al.Základní údaje
Originální název
Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection
Název česky
Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection
Autoři
HYRŠL, Pavel (203 Česká republika, garant, domácí), Emad IBRAHIM (818 Egypt), Pavel DOBEŠ (203 Česká republika, domácí), Martin KUNC (203 Česká republika, domácí) a Dalibor KODRÍK (203 Česká republika)
Vydání
25th European Drosophila Research Conference, 2017
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30102 Immunology
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/17:00095034
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
Drosophila; hlístice; hmyz; imunita; adipokineticý hormon
Klíčová slova anglicky
Drosophila; nematodes; immunity; insect; adipokinetic hormone
Změněno: 11. 12. 2017 09:48, Ing. Nicole Zrilić
V originále
Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system. It is known that they activate anti-stress defence where adipokinetic hormone (AKH), important stress hormone responsible for keeping homeostasis in insect body, and adenosine, a purine nucleotide that serves as signalling factor in anti-stress reaction, play a role. Drosophila melanogaster mutants with non-functional AKH (AKH-def) and impaired adenosine receptor (AdoR-def), and two nematodal species Steinernema carpocapsae and Heterorhabditis bacteriophora were used in our study. The results revealed that both mutants showed significantly lower mortality after infection than the control, and reversely, application of external AKH significantly increased the mortality. We have found that the AKH absence reduced production of nutrients into the haemolymph, which inhibited the development of infection, and vice versa the application of AKH restored production of energy rich metabolites and supported the infection. Indeed, our experiments indicated clear stimulatory role of AKH, and minor modulatory role of adenosine in regulation of the trehalose level in Drosophila haemolymph. The nematode infection was accompanied by higher production of AKH, by increasing of anti-oxidative activity and by raising of nutrients in haemolymph which affected also larval locomotion as we detected by FTIR-based tracking method. Supported by the grant 17-03253S (Czech Science Foundation) and by the Missions Department in Cairo, Egypt.
Česky
Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system. It is known that they activate anti-stress defence where adipokinetic hormone (AKH), important stress hormone responsible for keeping homeostasis in insect body, and adenosine, a purine nucleotide that serves as signalling factor in anti-stress reaction, play a role. Drosophila melanogaster mutants with non-functional AKH (AKH-def) and impaired adenosine receptor (AdoR-def), and two nematodal species Steinernema carpocapsae and Heterorhabditis bacteriophora were used in our study. The results revealed that both mutants showed significantly lower mortality after infection than the control, and reversely, application of external AKH significantly increased the mortality. We have found that the AKH absence reduced production of nutrients into the haemolymph, which inhibited the development of infection, and vice versa the application of AKH restored production of energy rich metabolites and supported the infection. Indeed, our experiments indicated clear stimulatory role of AKH, and minor modulatory role of adenosine in regulation of the trehalose level in Drosophila haemolymph. The nematode infection was accompanied by higher production of AKH, by increasing of anti-oxidative activity and by raising of nutrients in haemolymph which affected also larval locomotion as we detected by FTIR-based tracking method. Supported by the grant 17-03253S (Czech Science Foundation) and by the Missions Department in Cairo, Egypt.
Návaznosti
| GA17-03253S, projekt VaV |
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