Inflammatory changes in the choroid plexus after paclitaxel
treatment

k 2017

Inflammatory changes in the choroid plexus after paclitaxel treatment

SINAI CACODCAR, Rahul Sanjiva, Adriana SLANÍKOVÁ, Michael LUJC, Ilona KLUSÁKOVÁ, Petr DUBOVÝ et. al.

Základní údaje

Originální název

Inflammatory changes in the choroid plexus after paclitaxel treatment

Název česky

Zánětlivé změny choroidního plexu po léčbě paklitaxelem

Autoři

SINAI CACODCAR, Rahul Sanjiva, Adriana SLANÍKOVÁ, Michael LUJC, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL

Vydání

Morphology 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

978-80-263-1315-1

Klíčová slova česky

Paclitaxel, krevně-likvorová bariéra, plexus choroideus, neuropatie, makrofágy, TLR9 , ED1, ED2

Klíčová slova anglicky

Paclitaxel, blood-cerebrospinal barrier, plexus choroideus, neuropathy, macrophages, TLR9, ED1, ED2

Změněno: 5. 10. 2017 15:47, MUDr. Rahul Sanjiva Sinai Cacodcar

Anotace

V originále

Peripheral neuropathy as a side effect of chemotherapeutic agent Paclitaxel is caused by disorders of calcium homeostasis and mitochondrial damage in axons. These changes are associated with release of damage associated molecular patterns to the blood stream with potential effect on the blood-cerebrospinal fluid (B-CSF) barrier presented in choroid plexus (CP) of the brain ventricles. The aim of our work was to study the cellular and the molecular changes in the CP after intraperitoneal administration of Paclitaxel. Wistar rats (n=23, males) were used in our experiments. Intraperitoneal injection of Paclitaxel in 4 doses with cumulative dose 8 mg/kg was performed in experimental rats while control animals received vehiculum (alcohol and cremophor; 1:1). The animals were left to survive for 1, 3, 7 and 14 days from the last application. Experimental and control rats were sacrificed together with naïve rats and perfused transcardially by Zamboni´s fixative. Cryostat coronal sections through the brain were cut and immunostained for activated (ED1) and resident (ED2) macrophages as well as for Toll-like Receptors 9 (TLR-9). Numbers of ED1+ and ED2+ cells per 1 mm2 and intensity of TLR9 immunofluorescence in CP were counted and statistically analyzed. Presence of ED1+ and ED2+ macrophages was observed in epiplexus position of CP. Statistically significant increased numbers of ED1+ and ED2+ macrophages comparing to naïve and control rats were found in the CP after administration of Paclitaxel. Number of ED1+ cells increased with time of survival. TLR-9 immunofluorescence was presented in cytoplasm of the cuboidal cells and its immunofluorescence intensity increased with time after the last application. Our results clearly indicate that intraperitoneal administration of Paclitaxel induces inflammatory response in CP expressed by increased numbers of macrophages and expression of TLR-9 following the time from the last application.

Návaznosti

MUNI/A/0973/2016, interní kód MU

Název: Spoje a prostupnost hemato-likvorové bariéry po podání Paclitaxelu

Investor: Masarykova univerzita, Spoje a prostupnost hemato-likvorové bariéry po podání Paclitaxelu, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

SINAI CACODCAR, Rahul Sanjiva, Adriana SLANÍKOVÁ, Michael LUJC, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. Inflammatory changes in the choroid plexus after paclitaxel treatment. In Morphology 2017. 2017. ISBN 978-80-263-1315-1.

@proceedings{1391602,
   author = {Sinai Cacodcar, Rahul Sanjiva and Slaníková, Adriana and Lujc, Michael and Klusáková, Ilona and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2017},
   keywords = {Paclitaxel, blood-cerebrospinal barrier, plexus choroideus, neuropathy, macrophages, TLR9, ED1, ED2},
   language = {eng},
   isbn = {978-80-263-1315-1},
   title = {Inflammatory changes in the choroid plexus after paclitaxel treatment},
   year = {2017}
}

TY  - CONF
ID  - 1391602
AU  - Sinai Cacodcar, Rahul Sanjiva - Slaníková, Adriana - Lujc, Michael - Klusáková, Ilona - Dubový, Petr - Joukal, Marek
PY  - 2017
TI  - Inflammatory changes in the choroid plexus after paclitaxel treatment
SN  - 9788026313151
KW  - Paclitaxel, blood-cerebrospinal barrier, plexus choroideus, neuropathy, macrophages, TLR9, ED1, ED2
N2  - Peripheral neuropathy as a side effect of chemotherapeutic agent Paclitaxel is caused by disorders of calcium homeostasis and mitochondrial damage in axons. These changes are associated with release of damage associated molecular patterns to the blood stream with potential effect on the blood-cerebrospinal fluid (B-CSF) barrier presented in choroid plexus (CP) of the brain ventricles. The aim of our work was to study the cellular and the molecular changes in the CP after intraperitoneal administration of Paclitaxel. Wistar rats (n=23, males) were used in our experiments. Intraperitoneal injection of Paclitaxel in 4 doses with cumulative dose 8 mg/kg was performed in experimental rats while control animals received vehiculum (alcohol and cremophor; 1:1). The animals were left to survive for 1, 3, 7 and 14 days from the last application. Experimental and control rats were sacrificed together with naïve rats and perfused transcardially by Zamboni´s fixative. Cryostat coronal sections through the brain were cut and immunostained for activated (ED1) and resident (ED2) macrophages as well as for Toll-like Receptors 9 (TLR-9). Numbers of ED1+ and ED2+ cells per 1 mm2 and intensity of TLR9 immunofluorescence in CP were counted and statistically analyzed. Presence of ED1+ and ED2+ macrophages was observed in epiplexus position of CP. Statistically significant increased numbers of ED1+ and ED2+ macrophages comparing to naïve and control rats were found in the CP after administration of Paclitaxel. Number of ED1+ cells increased with time of survival. TLR-9 immunofluorescence was presented in cytoplasm of the cuboidal cells and its immunofluorescence intensity increased with time after the last application. Our results clearly indicate that intraperitoneal administration of Paclitaxel induces inflammatory response in CP expressed by increased numbers of macrophages and expression of TLR-9 following the time from the last application.
ER  -

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