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@article{1391715, author = {Fedorko, Michal and Juráček, Jaroslav and Staník, Michal and Svoboda, Marek and Poprach, Alexandr and Büchler, Tomáš and Pacík, Dalibor and Doležel, Jan and Slabý, Ondřej}, article_location = {Zagreb}, article_number = {2}, doi = {http://dx.doi.org/10.11613/BM.2017.043}, keywords = {renal cell carcinoma; urinary miRNAs; let-7; diagnostic biomarker}, language = {eng}, issn = {1330-0962}, journal = {Biochemia Medica}, title = {Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma}, volume = {27}, year = {2017} }
TY - JOUR ID - 1391715 AU - Fedorko, Michal - Juráček, Jaroslav - Staník, Michal - Svoboda, Marek - Poprach, Alexandr - Büchler, Tomáš - Pacík, Dalibor - Doležel, Jan - Slabý, Ondřej PY - 2017 TI - Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma JF - Biochemia Medica VL - 27 IS - 2 SP - 411-417 EP - 411-417 PB - CROATIAN SOC MEDICAL BIOCHEMISTS SN - 13300962 KW - renal cell carcinoma KW - urinary miRNAs KW - let-7 KW - diagnostic biomarker N2 - Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC. ER -
FEDORKO, Michal, Jaroslav JURÁČEK, Michal STANÍK, Marek SVOBODA, Alexandr POPRACH, Tomáš BÜCHLER, Dalibor PACÍK, Jan DOLEŽEL a Ondřej SLABÝ. Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma. \textit{Biochemia Medica}. Zagreb: CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, roč.~27, č.~2, s.~411-417. ISSN~1330-0962. Dostupné z: https://dx.doi.org/10.11613/BM.2017.043.
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