Circulating plasma cells in monoclonal gammopathies

J 2017

Circulating plasma cells in monoclonal gammopathies

BEZDĚKOVÁ, Renata, Miroslav PENKA, Roman HÁJEK a Lucie ŘÍHOVÁ

Základní údaje

Originální název

Circulating plasma cells in monoclonal gammopathies

Autoři

BEZDĚKOVÁ, Renata (203 Česká republika, domácí), Miroslav PENKA (203 Česká republika), Roman HÁJEK (203 Česká republika, domácí) a Lucie ŘÍHOVÁ (203 Česká republika, domácí)

Vydání

Klinická onkologie, Praha, Ambit Media, 2017, 0862-495X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/17:00097852

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.14735/amko20172S29

Klíčová slova anglicky

circulating plasma cells; flow cytometry; monoclonal gammopathies; plasma cell leukemia

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 3. 2018 17:41, Soňa Böhmová

Anotace

V originále

Background: Monoclonal gammopathies are characterized by presence of clonal plasma cells in the bone marrow, although peripheral blood circulating plasma cells can be found in a significant proportion of patients. The number of circulating plasma cells is an independent prognostic marker associated with shorter survival, but it can also help to predict early relapse. The reason and mechanism of plasma cell expansion from the bone marrow to enter peripheral blood is still not entirely clear, but possible changes in the expression of adhesion molecules are probably involved. Multiparametric flow cytometry allows simple and exact enumeration of circulating plasma cells in different types of cell suspensions, even in their low quantity. The phenotype profile and confirmation of clonality regarding to their bone marrow clonal counterparts should be verified as well. There is no uniform method used in clinical laboratories for circulating plasma cells analyses at this moment. Aim: Review is focused on use of multiparametric flow cytometry for circulating plasma cells analysis in peripheral blood. It is comparing possibilities of their detection by different methods and on clinical relevance of that assessment. The standardization of analyses is the main goal. Conclusion: Multiparametric flow cytometry is a very sensitive method for detection of circulating plasma cells, so using a standardized approach can lead to determination and implementation of the flow cytometry diagnostic threshold in plasma cell leukemia suspicious cases as well as in prognostication of monoclonal gammopathies patients. Moreover,analysis of plasma cells phenotypic profile could probably clarify their future behaviour.

Citovat

BEZDĚKOVÁ, Renata, Miroslav PENKA, Roman HÁJEK a Lucie ŘÍHOVÁ. Circulating plasma cells in monoclonal gammopathies. Klinická onkologie. Praha: Ambit Media, 2017, roč. 30, Suppl. 2, s. "2S29"-"2S34", 6 s. ISSN 0862-495X. Dostupné z: https://dx.doi.org/10.14735/amko20172S29.

@article{1392119,
   author = {Bezděková, Renata and Penka, Miroslav and Hájek, Roman and Říhová, Lucie},
   article_location = {Praha},
   article_number = {Suppl. 2},
   doi = {http://dx.doi.org/10.14735/amko20172S29},
   keywords = {circulating plasma cells; flow cytometry; monoclonal gammopathies; plasma cell leukemia},
   language = {eng},
   issn = {0862-495X},
   journal = {Klinická onkologie},
   title = {Circulating plasma cells in monoclonal gammopathies},
   volume = {30},
   year = {2017}
}

TY  - JOUR
ID  - 1392119
AU  - Bezděková, Renata - Penka, Miroslav - Hájek, Roman - Říhová, Lucie
PY  - 2017
TI  - Circulating plasma cells in monoclonal gammopathies
JF  - Klinická onkologie
VL  - 30
IS  - Suppl. 2
SP  - "2S29"-"2S34"
EP  - "2S29"-"2S34"
PB  - Ambit Media
SN  - 0862495X
KW  - circulating plasma cells
KW  - flow cytometry
KW  - monoclonal gammopathies
KW  - plasma cell leukemia
N2  - Background: Monoclonal gammopathies are characterized by presence of clonal plasma cells in the bone marrow, although peripheral blood circulating plasma cells can be found in a significant proportion of patients. The number of circulating plasma cells is an independent prognostic marker associated with shorter survival, but it can also help to predict early relapse. The reason and mechanism of plasma cell expansion from the bone marrow to enter peripheral blood is still not entirely clear, but possible changes in the expression of adhesion molecules are probably involved. Multiparametric flow cytometry allows simple and exact enumeration of circulating plasma cells in different types of cell suspensions, even in their low quantity. The phenotype profile and confirmation of clonality regarding to their bone marrow clonal counterparts should be verified as well. There is no uniform method used in clinical laboratories for circulating plasma cells analyses at this moment. Aim: Review is focused on use of multiparametric flow cytometry for circulating plasma cells analysis in peripheral blood. It is comparing possibilities of their detection by different methods and on clinical relevance of that assessment. The standardization of analyses is the main goal. Conclusion: Multiparametric flow cytometry is a very sensitive method for detection of circulating plasma cells, so using a standardized approach can lead to determination and implementation of the flow cytometry diagnostic threshold in plasma cell leukemia suspicious cases as well as in prognostication of monoclonal gammopathies patients. Moreover,analysis of plasma cells phenotypic profile could probably clarify their future behaviour.
ER  -

BEZDĚKOVÁ, Renata, Miroslav PENKA, Roman HÁJEK a Lucie ŘÍHOVÁ. Circulating plasma cells in monoclonal gammopathies. \textit{Klinická onkologie}. Praha: Ambit Media, 2017, roč.~30, Suppl. 2, s.~''2S29''-''2S34'', 6 s. ISSN~0862-495X. Dostupné z: https://dx.doi.org/10.14735/amko20172S29.

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