Investigation of deferiprone binding to different essential
metal ions using microscale thermophoresis and electrospray
ionization mass spectrometry

J 2018

Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry

ASMARI, Mufarreh; Lenka MICHALCOVÁ; Hassan A. ALHAZMI; Zdeněk GLATZ; Sami EL DEEB et al.

Základní údaje

Originální název

Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry

Autoři

ASMARI, Mufarreh; Lenka MICHALCOVÁ; Hassan A. ALHAZMI; Zdeněk GLATZ a Sami EL DEEB

Vydání

Microchemical Journal, Holandsko, Elsevier, 2018, 0026-265X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.206

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00100730

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

MST; MS; bio-kovy; deferiprone; CP20

Klíčová slova anglicky

Microscale thermophoresis; MS; biometals; deferiprone; CP20

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 10:46, Mgr. Michal Petr

Anotace

V originále

In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.

Návaznosti

GBP206/12/G014, projekt VaV

Název: Centrum pokročilých bioanalytických technologií

MUNI/A/1278/2016, interní kód MU

Název: Podpora biochemického výzkumu v roce 2017

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Přiložené soubory

Asmari_M_Investigation_of_deferiprone_binding_to_different_essential_metal.pdf

Požádat o autorskou verzi souboru

Citovat

ASMARI, Mufarreh; Lenka MICHALCOVÁ; Hassan A. ALHAZMI; Zdeněk GLATZ a Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. Microchemical Journal. Holandsko: Elsevier, 2018, roč. 2018, č. 137, s. 98-104. ISSN 0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2017.10.004.

@article{1392343,
   author = {Asmari, Mufarreh and Michalcová, Lenka and Alhazmi, Hassan A. and Glatz, Zdeněk and El Deeb, Sami},
   article_location = {Holandsko},
   article_number = {137},
   doi = {https://doi.org/10.1016/j.microc.2017.10.004},
   keywords = {Microscale thermophoresis; MS; biometals; deferiprone; CP20},
   language = {eng},
   issn = {0026-265X},
   journal = {Microchemical Journal},
   title = {Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry},
   url = {http://dx.doi.org/10.1016/j.microc.2017.10.004},
   volume = {2018},
   year = {2018}
}

TY  - JOUR
ID  - 1392343
AU  - Asmari, Mufarreh - Michalcová, Lenka - Alhazmi, Hassan A. - Glatz, Zdeněk - El Deeb, Sami
PY  - 2018
TI  - Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry
JF  - Microchemical Journal
VL  - 2018
IS  - 137
SP  - 98-104
EP  - 98-104
PB  - Elsevier
SN  - 0026265X
KW  - Microscale thermophoresis
KW  - MS
KW  - biometals
KW  - deferiprone
KW  - CP20
UR  - http://dx.doi.org/10.1016/j.microc.2017.10.004
N2  - In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.
ER  -

ASMARI, Mufarreh; Lenka MICHALCOVÁ; Hassan A. ALHAZMI; Zdeněk GLATZ a Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. \textit{Microchemical Journal}. Holandsko: Elsevier, 2018, roč.~2018, č.~137, s.~98-104. ISSN~0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2017.10.004.

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