KEJNOVSKÁ, Iva, Klára BEDNÁŘOVÁ, Daniel RENČIUK, Zuzana DVOŘÁKOVÁ, Petra ŠKOLÁKOVÁ, Lukáš TRANTÍREK, Radovan FIALA, Michaela VORLÍČKOVÁ a J. SAGI. Clustered abasic lesions profoundly change the structure and stability of human telomeric G-quadruplexes. Nucleic Acids Research. Oxford: Oxford University Press, 2017, roč. 45, č. 8, s. 4294-4305. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkx191. |
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@article{1392894, author = {Kejnovská, Iva and Bednářová, Klára and Renčiuk, Daniel and Dvořáková, Zuzana and Školáková, Petra and Trantírek, Lukáš and Fiala, Radovan and Vorlíčková, Michaela and Sagi, J.}, article_location = {Oxford}, article_number = {8}, doi = {http://dx.doi.org/10.1093/nar/gkx191}, keywords = {DNA-DAMAGE CLUSTERS; K+ SOLUTION; GUANINE QUADRUPLEXES; IONIZING-RADIATION; BASE LESIONS; CELLS; 8-OXOGUANINE; SEQUENCE; TETRAD; SITES}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {Clustered abasic lesions profoundly change the structure and stability of human telomeric G-quadruplexes}, url = {https://watermark.silverchair.com/gkx191.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbEwggGtBgkqhkiG9w0BBwagggGeMIIBmgIBADCCAZMGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMPhjm4qncd9c3wayHAgEQgIIBZHNy50Rs-TH7Fcsl3VTJ-F4kBqgbb6qbJ4tdfAMDkpZjURB}, volume = {45}, year = {2017} }
TY - JOUR ID - 1392894 AU - Kejnovská, Iva - Bednářová, Klára - Renčiuk, Daniel - Dvořáková, Zuzana - Školáková, Petra - Trantírek, Lukáš - Fiala, Radovan - Vorlíčková, Michaela - Sagi, J. PY - 2017 TI - Clustered abasic lesions profoundly change the structure and stability of human telomeric G-quadruplexes JF - Nucleic Acids Research VL - 45 IS - 8 SP - 4294-4305 EP - 4294-4305 PB - Oxford University Press SN - 03051048 KW - DNA-DAMAGE CLUSTERS KW - K+ SOLUTION KW - GUANINE QUADRUPLEXES KW - IONIZING-RADIATION KW - BASE LESIONS KW - CELLS KW - 8-OXOGUANINE KW - SEQUENCE KW - TETRAD KW - SITES UR - https://watermark.silverchair.com/gkx191.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbEwggGtBgkqhkiG9w0BBwagggGeMIIBmgIBADCCAZMGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMPhjm4qncd9c3wayHAgEQgIIBZHNy50Rs-TH7Fcsl3VTJ-F4kBqgbb6qbJ4tdfAMDkpZjURB L2 - https://watermark.silverchair.com/gkx191.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbEwggGtBgkqhkiG9w0BBwagggGeMIIBmgIBADCCAZMGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMPhjm4qncd9c3wayHAgEQgIIBZHNy50Rs-TH7Fcsl3VTJ-F4kBqgbb6qbJ4tdfAMDkpZjURB N2 - Ionizing radiation produces clustered damage to DNA which is difficult to repair and thus more harmful than single lesions. Clustered lesions have only been investigated in dsDNA models. Introducing the term 'clustered damage to G-quadruplexes' we report here on the structural effects of multiple tetrahydrofuranyl abasic sites replacing loop adenines (A/AP) and tetrad guanines (G/AP) in quadruplexes formed by the human telomere d[AG(3)(TTAG(3))(3)] (htel-22) and d[TAG(3)(TTAG(3))(3)TT] (htel-25) in K+ solutions. Single to triple A/APs increased the population of parallel strands in their structures by stabilizing propeller type loops, shifting the antiparallel htel-22 into hybrid or parallel quadruplexes. In htel-25, the G/APs inhibited the formation of parallel strands and these adopted antiparallel topologies. Clustered G/AP and A/APs reduced the thermal stability of the wild-type htel-25. Depending on position, A/APs diminished or intensified the damaging effect of the G/APs. Taken together, clustered lesions can disrupt the topology and stability of the htel quadruplexes and restrict their conformational space. These in vitro results suggest that formation of clustered lesions in the chromosome capping structure can result in the unfolding of existing G-quadruplexes which can lead to telomere shortening. ER -
KEJNOVSKÁ, Iva, Klára BEDNÁŘOVÁ, Daniel RENČIUK, Zuzana DVOŘÁKOVÁ, Petra ŠKOLÁKOVÁ, Lukáš TRANTÍREK, Radovan FIALA, Michaela VORLÍČKOVÁ a J. SAGI. Clustered abasic lesions profoundly change the structure and stability of human telomeric G-quadruplexes. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2017, roč.~45, č.~8, s.~4294-4305. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkx191.
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