Formation of Staphylococcus aureus biofilm in the presence of
sub-lethal concentrations of disinfectants: a transcriptomic
analysis using RNA-seq

J 2017

Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

SLANÝ, Michal, Jan OPPELT a Lenka ČINČÁROVÁ

Základní údaje

Originální název

Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Autoři

SLANÝ, Michal (203 Česká republika), Jan OPPELT (203 Česká republika, garant, domácí) a Lenka ČINČÁROVÁ (203 Česká republika)

Vydání

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, American Society for Microbiology, 2017, 0099-2240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.633

Kód RIV

RIV/00216224:14740/17:00098003

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1128/AEM.01643-17

UT WoS

000416937000008

Klíčová slova anglicky

STRESS RESISTANCE; QUALITY-CONTROL; EXPRESSION; BIOCONDUCTOR; PREVALENCE; REGULATORS; STRATEGIES; VIRULENCE; CATALASE; SAMPLES

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 5. 2018 14:09, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Staphylococcus aureus is a common biofilm-forming pathogen. Low doses of disinfectants have previously been reported to promote biofilm formation and to increase virulence. The aim of this study was to use RNA-seq analysis to investigate global transcriptional changes in S. aureus in response to sub-lethal concentrations of commonly used food industry disinfectants (ethanol, Et; chloramine T, ChT; and their combination, Et_ChT), in order to better understand the effects of these agents on biofilm formation. Treatment with Et and Et_ChT resulted in more significantly altered expression profiles, compared to ChT. Our results revealed that Et and Et_ChT treatments enhanced the expression of genes responsible for regulation of gene expression (sigB), cell surface factors (clfAB), adhesins (sdrDE) and capsular polysaccharides (cap8EFGL) resulting in more intact biofilm. In addition, in this study we were able to identify the pathways involved in the adaptation of S. aureus to the stress of ChT treatment. Further, Et suppressed the effect of ChT on gene expression when these agents were used together at sub-lethal concentrations. These data show that in the presence of sub-lethal concentrations of tested disinfectants, S. aureus cells triggers protective mechanisms and tries to cope with them.

Návaznosti

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

SLANÝ, Michal, Jan OPPELT a Lenka ČINČÁROVÁ. Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, 2017, roč. 83, č. 24, s. nestránkováno, 13 s. ISSN 0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.01643-17.

@article{1393159,
   author = {Slaný, Michal and Oppelt, Jan and Činčárová, Lenka},
   article_number = {24},
   doi = {http://dx.doi.org/10.1128/AEM.01643-17},
   keywords = {STRESS RESISTANCE; QUALITY-CONTROL; EXPRESSION; BIOCONDUCTOR; PREVALENCE; REGULATORS; STRATEGIES; VIRULENCE; CATALASE; SAMPLES},
   language = {eng},
   issn = {0099-2240},
   journal = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
   title = {Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq},
   url = {http://aem.asm.org/content/early/2017/10/09/AEM.01643-17},
   volume = {83},
   year = {2017}
}

TY  - JOUR
ID  - 1393159
AU  - Slaný, Michal - Oppelt, Jan - Činčárová, Lenka
PY  - 2017
TI  - Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq
JF  - APPLIED AND ENVIRONMENTAL MICROBIOLOGY
VL  - 83
IS  - 24
SP  - nestránkováno
EP  - nestránkováno
PB  - American Society for Microbiology
SN  - 00992240
KW  - STRESS RESISTANCE
KW  - QUALITY-CONTROL
KW  - EXPRESSION
KW  - BIOCONDUCTOR
KW  - PREVALENCE
KW  - REGULATORS
KW  - STRATEGIES
KW  - VIRULENCE
KW  - CATALASE
KW  - SAMPLES
UR  - http://aem.asm.org/content/early/2017/10/09/AEM.01643-17
L2  - http://aem.asm.org/content/early/2017/10/09/AEM.01643-17
N2  - Staphylococcus aureus is a common biofilm-forming pathogen. Low doses of disinfectants have previously been reported to promote biofilm formation and to increase virulence. The aim of this study was to use RNA-seq analysis to investigate global transcriptional changes in S. aureus in response to sub-lethal concentrations of commonly used food industry disinfectants (ethanol, Et; chloramine T, ChT; and their combination, Et_ChT), in order to better understand the effects of these agents on biofilm formation. Treatment with Et and Et_ChT resulted in more significantly altered expression profiles, compared to ChT. Our results revealed that Et and Et_ChT treatments enhanced the expression of genes responsible for regulation of gene expression (sigB), cell surface factors (clfAB), adhesins (sdrDE) and capsular polysaccharides (cap8EFGL) resulting in more intact biofilm. In addition, in this study we were able to identify the pathways involved in the adaptation of S. aureus to the stress of ChT treatment. Further, Et suppressed the effect of ChT on gene expression when these agents were used together at sub-lethal concentrations. These data show that in the presence of sub-lethal concentrations of tested disinfectants, S. aureus cells triggers protective mechanisms and tries to cope with them.
ER  -

SLANÝ, Michal, Jan OPPELT a Lenka ČINČÁROVÁ. Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq. \textit{APPLIED AND ENVIRONMENTAL MICROBIOLOGY}. American Society for Microbiology, 2017, roč.~83, č.~24, s.~nestránkováno, 13 s. ISSN~0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.01643-17.

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