| BELICKY, Stefan, Hana CERNOCKA, Tomas BERTOK, Alena HOLAZOVA, Kamila RÉBLOVÁ, Emil PALECEK, Jan TKAC a Veronika OSTATNA. Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins. Bioelectrochemistry. SWITZERLAND: Elsevier Science SA, 2017, roč. 117, October, s. 89-94. ISSN 1567-5394. Dostupné z: https://dx.doi.org/10.1016/j.bioelechem.2017.06.005. |
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@article{1394044,
author = {Belicky, Stefan and Cernocka, Hana and Bertok, Tomas and Holazova, Alena and Réblová, Kamila and Palecek, Emil and Tkac, Jan and Ostatna, Veronika},
article_location = {SWITZERLAND},
article_number = {October},
doi = {http://dx.doi.org/10.1016/j.bioelechem.2017.06.005},
keywords = {A prostate specific antigen; Lectin-glycoprotein interaction; Sialylated glycan isomers; Chronopotentiometric analysis; Mercury electrode},
language = {eng},
issn = {1567-5394},
journal = {Bioelectrochemistry},
title = {Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins},
url = {http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub},
volume = {117},
year = {2017}
}
TY - JOUR
ID - 1394044
AU - Belicky, Stefan - Cernocka, Hana - Bertok, Tomas - Holazova, Alena - Réblová, Kamila - Palecek, Emil - Tkac, Jan - Ostatna, Veronika
PY - 2017
TI - Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins
JF - Bioelectrochemistry
VL - 117
IS - October
SP - 89-94
EP - 89-94
PB - Elsevier Science SA
SN - 15675394
KW - A prostate specific antigen
KW - Lectin-glycoprotein interaction
KW - Sialylated glycan isomers
KW - Chronopotentiometric analysis
KW - Mercury electrode
UR - http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub
L2 - http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub
N2 - In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA). Incubation of PSA-modified electrode with specific SNA lectin resulted in an increase of CPS peak H of the complex as compared to this peak of individual PSA. By adjusting polarization current and temperature, PSA-MAA interaction can be either eliminated or distinguished from the more abundant PSA-SNA complex. CPS data were in a good agreement with the data obtained by complementary methods, namely surface plasmon resonance and fluorescent lectin microarray. It can be anticipated that CPS will find application in glycomics and proteomics. (C) 2017 Elsevier B.V. All tights reserved.
ER -
BELICKY, Stefan, Hana CERNOCKA, Tomas BERTOK, Alena HOLAZOVA, Kamila RÉBLOVÁ, Emil PALECEK, Jan TKAC a Veronika OSTATNA. Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins. \textit{Bioelectrochemistry}. SWITZERLAND: Elsevier Science SA, 2017, roč.~117, October, s.~89-94. ISSN~1567-5394. Dostupné z: https://dx.doi.org/10.1016/j.bioelechem.2017.06.005.
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