A New Two-Step Chromatographic Procedure for Fractionation of
Potato Proteins with Potato Fruit Juice and Spray-Dried Protein
as Source Materials

J 2017

A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials

SCHMIDT, Jesper Malling, Mathias GREVE-POULSEN, Henriette DAMGAARD, Anne Vuholm SUNDS, Zbyněk ZDRÁHAL et. al.

Základní údaje

Originální název

A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials

Autoři

SCHMIDT, Jesper Malling (208 Dánsko), Mathias GREVE-POULSEN (208 Dánsko), Henriette DAMGAARD (208 Dánsko), Anne Vuholm SUNDS (208 Dánsko), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), Marianne HAMMERSHOJ (208 Dánsko) a Lotte Bach LARSEN (208 Dánsko)

Vydání

Food and Bioprocess Technology, New York, Springer, 2017, 1935-5130

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.998

Kód RIV

RIV/00216224:14740/17:00098176

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s11947-017-1966-4

UT WoS

000412021800002

Klíčová slova anglicky

Potato protein; Patatin; Protease inhibitor; Polyphenol oxidase; Glycoalkaloids; Chlorogenic acid

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 3. 2018 16:23, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

In an effort to purify potato proteins of superior food grade quality, a new procedure involving anion exchange (ion exchange (IEX)) and hydrophobic interaction chromatography (HIC) was established. Liquid potato fruit juice (PFJ) or re-suspended spray-dried protein was separated by IEX yielding two fractions: a protease inhibitor (PI)-rich fraction and a patatin-rich fraction. Each of these fractions was re-chromatographed on HIC, resulting in two new sub-fractions which were characterised by electrophoresis and mass spectrometry. A high-quality powder should have high lightness, low polyphenol oxidase (PPO) activity and glycoalkaloid content below 150 mu g/g. The PI fraction from spray-dried powder had high lightness (L* = 83) compared to patatin (L* = 50), whereas IEX purification from PFJ resulted in a PI fraction with decreased lightness (L* = 66) and a patatin fraction with increased lightness (L* = 68). HIC fractionation led to increased lightness for patatin fraction, but decreased lightness for the PFJ PI fractions. HIC purification significantly lowered PPO activity in the fractions due to its selective affinity. The lowest total glycoalkaloid content was generally found in HIC fractions from spray-dried powder (150 mu g/g), while high levels (> 1000 mu g/g) were found in PI fractions from PFJ. In conclusion, it was possible to obtain a PI-rich protein isolate from powder with good-quality attributes after both IEX and HIC, while for patatin, the best quality was obtained after the HIC only, and there, the colour or PPO activity may still be a problem depending on source material.

Návaznosti

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SCHMIDT, Jesper Malling, Mathias GREVE-POULSEN, Henriette DAMGAARD, Anne Vuholm SUNDS, Zbyněk ZDRÁHAL, Marianne HAMMERSHOJ a Lotte Bach LARSEN. A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials. Food and Bioprocess Technology. New York: Springer, 2017, roč. 10, č. 11, s. 1946-1958. ISSN 1935-5130. Dostupné z: https://dx.doi.org/10.1007/s11947-017-1966-4.

@article{1394840,
   author = {Schmidt, Jesper Malling and GreveandPoulsen, Mathias and Damgaard, Henriette and Sunds, Anne Vuholm and Zdráhal, Zbyněk and Hammershoj, Marianne and Larsen, Lotte Bach},
   article_location = {New York},
   article_number = {11},
   doi = {http://dx.doi.org/10.1007/s11947-017-1966-4},
   keywords = {Potato protein; Patatin; Protease inhibitor; Polyphenol oxidase; Glycoalkaloids; Chlorogenic acid},
   language = {eng},
   issn = {1935-5130},
   journal = {Food and Bioprocess Technology},
   title = {A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials},
   volume = {10},
   year = {2017}
}

TY  - JOUR
ID  - 1394840
AU  - Schmidt, Jesper Malling - Greve-Poulsen, Mathias - Damgaard, Henriette - Sunds, Anne Vuholm - Zdráhal, Zbyněk - Hammershoj, Marianne - Larsen, Lotte Bach
PY  - 2017
TI  - A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials
JF  - Food and Bioprocess Technology
VL  - 10
IS  - 11
SP  - 1946-1958
EP  - 1946-1958
PB  - Springer
SN  - 19355130
KW  - Potato protein
KW  - Patatin
KW  - Protease inhibitor
KW  - Polyphenol oxidase
KW  - Glycoalkaloids
KW  - Chlorogenic acid
N2  - In an effort to purify potato proteins of superior food grade quality, a new procedure involving anion exchange (ion exchange (IEX)) and hydrophobic interaction chromatography (HIC) was established. Liquid potato fruit juice (PFJ) or re-suspended spray-dried protein was separated by IEX yielding two fractions: a protease inhibitor (PI)-rich fraction and a patatin-rich fraction. Each of these fractions was re-chromatographed on HIC, resulting in two new sub-fractions which were characterised by electrophoresis and mass spectrometry. A high-quality powder should have high lightness, low polyphenol oxidase (PPO) activity and glycoalkaloid content below 150 mu g/g. The PI fraction from spray-dried powder had high lightness (L* = 83) compared to patatin (L* = 50), whereas IEX purification from PFJ resulted in a PI fraction with decreased lightness (L* = 66) and a patatin fraction with increased lightness (L* = 68). HIC fractionation led to increased lightness for patatin fraction, but decreased lightness for the PFJ PI fractions. HIC purification significantly lowered PPO activity in the fractions due to its selective affinity. The lowest total glycoalkaloid content was generally found in HIC fractions from spray-dried powder (150 mu g/g), while high levels (> 1000 mu g/g) were found in PI fractions from PFJ. In conclusion, it was possible to obtain a PI-rich protein isolate from powder with good-quality attributes after both IEX and HIC, while for patatin, the best quality was obtained after the HIC only, and there, the colour or PPO activity may still be a problem depending on source material.
ER  -

SCHMIDT, Jesper Malling, Mathias GREVE-POULSEN, Henriette DAMGAARD, Anne Vuholm SUNDS, Zbyněk ZDRÁHAL, Marianne HAMMERSHOJ a Lotte Bach LARSEN. A New Two-Step Chromatographic Procedure for Fractionation of Potato Proteins with Potato Fruit Juice and Spray-Dried Protein as Source Materials. \textit{Food and Bioprocess Technology}. New York: Springer, 2017, roč.~10, č.~11, s.~1946-1958. ISSN~1935-5130. Dostupné z: https://dx.doi.org/10.1007/s11947-017-1966-4.

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