Lungosphere Assay: 3D Culture of Lung Epithelial
Stem/Progenitor Cells

C 2017

Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

RABATA, Anas, Aleš HAMPL a Zuzana KOLEDOVÁ

Základní údaje

Originální název

Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Autoři

RABATA, Anas (760 Sýrie, domácí), Aleš HAMPL (203 Česká republika, domácí) a Zuzana KOLEDOVÁ (703 Slovensko, garant, domácí)

Vydání

1st. ed. New York, 3D Cell Culture : Methods and Protocols, od s. 149-165, 17 s. Methods in Molecular Biology, volume 1612, 2017

Nakladatel

Humana Press

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14110/17:00095147

Organizační jednotka

Lékařská fakulta

ISBN

978-1-4939-7019-3

DOI

http://dx.doi.org/10.1007/978-1-4939-7021-6_11

UT WoS

000447757200012

Klíčová slova anglicky

3D culture; Lung epithelial stem/progenitor cells; Lungosphere assay; Spheroid culture

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 4. 2020 09:05, Mgr. Tereza Miškechová

Anotace

V originále

Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.

Návaznosti

GJ16-20031Y, projekt VaV

Název: Signalizace FGF ve fibroblastech mléčné žlázy a její úloha ve vývoji a nádorech mléčné žlázy

Investor: Grantová agentura ČR, Signalizace FGF ve fibroblastech mléčné žlázy a její úloha ve vývoji a nádorech mléčné žlázy

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

ROZV/20/LF/2015, interní kód MU

Název: LF - Příspěvek IP 2015

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2015

Citovat

RABATA, Anas, Aleš HAMPL a Zuzana KOLEDOVÁ. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells. In Koledova Zuzana. 3D Cell Culture : Methods and Protocols. 1st. ed. New York: Humana Press, 2017, s. 149-165. Methods in Molecular Biology, volume 1612. ISBN 978-1-4939-7019-3. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-7021-6_11.

@inbook{1394952,
   author = {Rabata, Anas and Hampl, Aleš and Koledová, Zuzana},
   address = {New York},
   booktitle = {3D Cell Culture : Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-7021-6_11},
   edition = {1st. ed.},
   editor = {Koledova Zuzana},
   keywords = {3D culture; Lung epithelial stem/progenitor cells; Lungosphere assay; Spheroid culture},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-4939-7019-3},
   pages = {149-165},
   publisher = {Humana Press},
   title = {Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells},
   url = {https://link.springer.com/protocol/10.1007/978-1-4939-7021-6_11},
   year = {2017}
}

TY  - CHAP
ID  - 1394952
AU  - Rabata, Anas - Hampl, Aleš - Koledová, Zuzana
PY  - 2017
TI  - Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
VL  - Methods in Molecular Biology, volume 1612
PB  - Humana Press
CY  - New York
SN  - 9781493970193
KW  - 3D culture
KW  - Lung epithelial stem/progenitor cells
KW  - Lungosphere assay
KW  - Spheroid culture
UR  - https://link.springer.com/protocol/10.1007/978-1-4939-7021-6_11
L2  - https://link.springer.com/protocol/10.1007/978-1-4939-7021-6_11
N2  - Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.
ER  -

RABATA, Anas, Aleš HAMPL a Zuzana KOLEDOVÁ. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells. In Koledova Zuzana. \textit{3D Cell Culture : Methods and Protocols}. 1st. ed. New York: Humana Press, 2017, s.~149-165. Methods in Molecular Biology, volume 1612. ISBN~978-1-4939-7019-3. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-7021-6\_{}11.

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