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@inbook{1395479, author = {Jaroš, Josef and Petrov, Michal and Tesařová, Markéta and Hampl, Aleš}, address = {New York}, booktitle = {3D Cell Culture : Methods and Protocols}, doi = {http://dx.doi.org/10.1007/978-1-4939-7021-6_30}, edition = {1st. ed.}, editor = {Zuzana Koledova}, keywords = {3D visualization; Image reconstruction; Image segmentation; Morphology; Organoid; SBF-SEM; Scanning electron microscopy; Serial block-face; Spheroid; Stem cell; Ultrastructure}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York}, isbn = {978-1-4939-7019-3}, pages = {417-431}, publisher = {Humana Press}, title = {Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy}, url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30}, year = {2017} }
TY - CHAP ID - 1395479 AU - Jaroš, Josef - Petrov, Michal - Tesařová, Markéta - Hampl, Aleš PY - 2017 TI - Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy VL - Methods in Molecular Biology, volume 1612 PB - Humana Press CY - New York SN - 9781493970193 KW - 3D visualization KW - Image reconstruction KW - Image segmentation KW - Morphology KW - Organoid KW - SBF-SEM KW - Scanning electron microscopy KW - Serial block-face KW - Spheroid KW - Stem cell KW - Ultrastructure UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30 L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30 N2 - Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures ER -
JAROŠ, Josef, Michal PETROV, Markéta TESAŘOVÁ a Aleš HAMPL. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy. In Zuzana Koledova. \textit{3D Cell Culture : Methods and Protocols}. 1st. ed. New York: Humana Press, 2017, s.~417-431. Methods in Molecular Biology, volume 1612. ISBN~978-1-4939-7019-3. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-7021-6\_{}30.
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