BUDNA, J., A. BRYJA, P. CELICHOWSKI, R. KAHAN, W. KRANC, S. CIESIOLKA, M. RYBSKA, S. BORYS, Michal JEŠETA, D. BUKOWSKA, P. ANTOSIK, K.P. BRUSSOW, M. BRUSKA, M. NOWICKI, M. ZABEL and B. KEMPISTY. Genes of cellular components of morphogenesis in porcine oocytes before and after IVM. REPRODUCTION. BRISTOL: BIOSCIENTIFICA LTD, 2017, vol. 154, No 4, p. 535-545. ISSN 1470-1626. Available from: https://dx.doi.org/10.1530/REP-17-0367.
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Basic information
Original name Genes of cellular components of morphogenesis in porcine oocytes before and after IVM
Authors BUDNA, J. (616 Poland), A. BRYJA (616 Poland), P. CELICHOWSKI (616 Poland), R. KAHAN (616 Poland), W. KRANC (616 Poland), S. CIESIOLKA (616 Poland), M. RYBSKA (616 Poland), S. BORYS (616 Poland), Michal JEŠETA (203 Czech Republic, guarantor, belonging to the institution), D. BUKOWSKA (616 Poland), P. ANTOSIK (616 Poland), K.P. BRUSSOW (616 Poland), M. BRUSKA (616 Poland), M. NOWICKI (616 Poland), M. ZABEL (616 Poland) and B. KEMPISTY (616 Poland).
Edition REPRODUCTION, BRISTOL, BIOSCIENTIFICA LTD, 2017, 1470-1626.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10605 Developmental biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.086
RIV identification code RIV/00216224:14110/17:00098260
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1530/REP-17-0367
UT WoS 000412579500021
Keywords in English oocyte
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 21/3/2018 16:10.
Abstract
Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular components of morphogenesis within the developing oocyte. Brilliant cresyl blue (BCB) was used to determine the developmental capability of porcine oocytes. The immature oocytes (GV stage) were compared with matured oocytes (MII stage), using microarray and qRT-PCR analysis to track changes in the genetic expression profile of transcriptome genes. The data showed substantial upregulation of genes influencing oocyte's morphology, cellular migration and adhesion, intracellular communication, as well as plasticity of nervous system. Conversely, downregulation involved genes related to microtubule reorganization, regulation of adhesion, proliferation, migration and cell differentiation processes in oocytes. This suggests that most genes recruited in morphogenesis in porcine oocyte in vitro, may have cellular maturational capability, since they have a higher level of expression before the oocyte's matured form. It shows the process of oocyte maturation and developmental capacity is orchestrated by significant cellular modifications during morphogenesis.
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