Methods of Candida dubliniensis identification and its
occurrence in human clinical material

J 2017

Methods of Candida dubliniensis identification and its occurrence in human clinical material

MAHELOVÁ, Martina and Filip RŮŽIČKA

Basic information

Original name

Methods of Candida dubliniensis identification and its occurrence in human clinical material

Authors

MAHELOVÁ, Martina (203 Czech Republic, belonging to the institution) and Filip RŮŽIČKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Folia microbiologica, Praha, Mikrobiologický ústav Praha AV ČR, 2017, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.311

RIV identification code

RIV/00216224:14110/17:00095672

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s12223-017-0510-2

UT WoS

000409167900005

Keywords in English

Candida dubliniensis

Abstract

V originále

Candida dubliniensis was reported as a new species in 1995. This species is often misidentified as Candida albicans. The aims of this work were to determine the occurrence of C. dubliniensis in various clinical materials, to evaluate several ways to identify it and to examine the genetic variability of isolates. Among 7706 isolates originally identified as C. albicans, 237 were identified as C. dubliniensis (3.1%). Most of the C. dubliniensis isolates were obtained from the upper and lower respiratory tract (61.4 and 22.9%). Five phenotypic methods including latex agglutination were used (cultivation on CHROMagar Candida, on Staib agar, at 42 A degrees C and in medium with 6.5% NaCl), but only cultivation on the medium with an increased concentration of NaCl and latex agglutination gave reliable results. Species-specific polymerase chain reaction was used as the confirmation method. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry provided less reliable results. In fact, 78.9% of C. dubliniensis isolates had scores above 1.7. However, the rest of them (21.1%) were also identified as C. dubliniensis even when the scores were lower than 1.7. Divergences among C. dubliniensis strains were evaluated by means of pulsed-field gel electrophoresis. Eighty-six selected C. dubliniensis isolates showed a 69.6% level of similarity. The results of this study expand the knowledge of the incidence, means of identification and genotypic divergence of C. dubliniensis isolates.

Links

LG15056, research and development project

Name: Pokračování činnosti v pozici ambasadora pro Českou republiku v rámci Americké společnosti pro mikrobiologii a (ASM) (Acronym: pacrasm)

Investor: Ministry of Education, Youth and Sports of the CR

NV16-31593A, research and development project

Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

MAHELOVÁ, Martina and Filip RŮŽIČKA. Methods of Candida dubliniensis identification and its occurrence in human clinical material. Folia microbiologica. Praha: Mikrobiologický ústav Praha AV ČR, 2017, vol. 62, No 5, p. 401-408. ISSN 0015-5632. Available from: https://dx.doi.org/10.1007/s12223-017-0510-2.

@article{1395641,
   author = {Mahelová, Martina and Růžička, Filip},
   article_location = {Praha},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s12223-017-0510-2},
   keywords = {Candida dubliniensis},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia microbiologica},
   title = {Methods of Candida dubliniensis identification and its occurrence in human clinical material},
   volume = {62},
   year = {2017}
}

TY  - JOUR
ID  - 1395641
AU  - Mahelová, Martina - Růžička, Filip
PY  - 2017
TI  - Methods of Candida dubliniensis identification and its occurrence in human clinical material
JF  - Folia microbiologica
VL  - 62
IS  - 5
SP  - 401-408
EP  - 401-408
PB  - Mikrobiologický ústav Praha AV ČR
SN  - 00155632
KW  - Candida dubliniensis
N2  - Candida dubliniensis was reported as a new species in 1995. This species is often misidentified as Candida albicans. The aims of this work were to determine the occurrence of C. dubliniensis in various clinical materials, to evaluate several ways to identify it and to examine the genetic variability of isolates. Among 7706 isolates originally identified as C. albicans, 237 were identified as C. dubliniensis (3.1%). Most of the C. dubliniensis isolates were obtained from the upper and lower respiratory tract (61.4 and 22.9%). Five phenotypic methods including latex agglutination were used (cultivation on CHROMagar Candida, on Staib agar, at 42 A degrees C and in medium with 6.5% NaCl), but only cultivation on the medium with an increased concentration of NaCl and latex agglutination gave reliable results. Species-specific polymerase chain reaction was used as the confirmation method. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry provided less reliable results. In fact, 78.9% of C. dubliniensis isolates had scores above 1.7. However, the rest of them (21.1%) were also identified as C. dubliniensis even when the scores were lower than 1.7. Divergences among C. dubliniensis strains were evaluated by means of pulsed-field gel electrophoresis. Eighty-six selected C. dubliniensis isolates showed a 69.6% level of similarity. The results of this study expand the knowledge of the incidence, means of identification and genotypic divergence of C. dubliniensis isolates.
ER  -

MAHELOVÁ, Martina and Filip RŮŽIČKA. Methods of Candida dubliniensis identification and its occurrence in human clinical material. \textit{Folia microbiologica}. Praha: Mikrobiologický ústav Praha AV ČR, 2017, vol.~62, No~5, p.~401-408. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-017-0510-2.

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