Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis

SLEZÁKOVÁ, Simona, Petra BOŘILOVÁ LINHARTOVÁ, Jiřina BÁRTOVÁ, Jitka PETANOVÁ, Pavel KUKLÍNEK, Antonín FASSMANN and Lydie IZAKOVIČOVÁ HOLLÁ. Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis. In XVIII. Setkání biochemiků a molekulárních biologů, Brno. 2017. ISBN 978-80-210-8765-1.

Basic information

• Original name: Association of matrix metalloproteinases gene variants with recurrent aphthous stomatitis
• Authors: SLEZÁKOVÁ, Simona (203 Czech Republic, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, belonging to the institution), Jiřina BÁRTOVÁ (203 Czech Republic), Jitka PETANOVÁ (203 Czech Republic), Pavel KUKLÍNEK (203 Czech Republic, belonging to the institution), Antonín FASSMANN (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution).
• Edition: XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30208 Dentistry, oral surgery and medicine
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/17:00100753
• Organization unit: Faculty of Medicine
• ISBN: 978-80-210-8765-1
• Keywords in English: matrix metalloproteinase; association studies; polymorphism; recurrent aphthous stomatitis

Abstract

Background: Recurrent aphthous stomatitis (RAS) is a common inflammatory disease of oral mucosa manifested by erosions and ulcers. Variability in genes coding matrix metalloproteinases (MMPs), the proteolytic enzymes modulating inflammation, has been previously associated with RAS. The aim of our study was to investigate six single nucleotide polymorphisms (SNPs) in genes for gelatinases (MMP2, MMP9) and stromelysin (MMP3) in patients with RAS and healthy controls. Subjects and methods: Totally, 226 subjects were enrolled in this case-control study: 71 patients with RAS and 155 healthy individuals were genotyped for SNPs in the MMP2 (rs243865, rs2287074), MMP3 (rs679620, rs35068180) and MMP9 (rs3918242, rs17576) genes by TaqMan qPCR or by PCR with restriction analysis. Results: While allele frequencies of rs3918242 MMP9 polymorphism and haplotype MMP9 (rs3918242, rs17576) were marginally significantly different between patients with RAS and healthy controls (P=0.04 and P=0.06, respectively), distribution of allele, genotype and haplotype frequencies of the other studied SNPs were found similar in both groups. Nevertheless, the period of ulcer healing significantly less frequently exceeded one week in the MMP3 (rs35068180) 6A/6A carriers and MMP3 (rs679620) CC carriers compared to other genotypes (P=0.01 for both). Conclusions: Our study suggests that MMP9 gene variability, affecting MMP9 expression and activity, may be involved in the development of RAS in the Czech population. Moreover, MMP3 variants could influence the duration of ulcer healing.

Links

• GB14-37368G, research and development project: Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

• MUNI/A/0948/2016, interní kód MU: Name: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba

Investor: Masaryk University, Category A

• NV15-29336A, research and development project: Name: Změny imunologické reaktivity u pacientů s recidivujícími aftami