The capability of minor quaternary benzophenanthridine
alkaloids to inhibit TNF-alpha secretion and cyclooxygenase
activity

J 2017

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity

HOŠEK, Jan, Kristýna ŠEBRLOVÁ, Petra KAUCKÁ, Ondřej PEŠ, Eva TÁBORSKÁ et. al.

Základní údaje

Originální název

The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity

Autoři

HOŠEK, Jan (203 Česká republika), Kristýna ŠEBRLOVÁ (203 Česká republika, domácí), Petra KAUCKÁ (203 Česká republika), Ondřej PEŠ (203 Česká republika, garant, domácí) a Eva TÁBORSKÁ (203 Česká republika, domácí)

Vydání

Acta Veterinaria Brno, Brno, Veterinární a farmaceutická univerzita Brno, 2017, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40300 4.3 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.422

Kód RIV

RIV/00216224:14110/17:00098458

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2754/avb201786030223

UT WoS

000416306200004

Klíčová slova anglicky

Cyclooxygenase; cytotoxicity; inflammation; TNF-alpha.

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 18:44, Soňa Böhmová

Anotace

V originále

Quaternary benzophenanthridine alkaloids are known to have a wide range of biological effects, including antimicrobial, antifungal, anti-inflammatory, and antitumour activities. However, only sanguinarine and chelerythrine have been studied intensively. The aim of this study was to evaluate the anti-inflammatory potential of the five minor quaternary benzophenanthridine alkaloids sanguilutine, sanguirubine, chelirubine, chelilutine, and macarpine in vitro and to compare them with more thoroughly studied sanguinarine and chelerythrine. Before making cell-based assays, the cytotoxicity of the alkaloids was evaluated. The anti-inflammatory potential of the chosen alkaloids was evaluated as for their ability to modulate the lipopolysaccharide-induced secretion of tumour necrosis factor alpha (TNF-alpha) in the macrophage-like cell line THP-1. The cyclooxygenase (COX)-1 and COX-2 inhibitory activities were also measured. The results indicate that the presence of a methylenedioxy ring attached at carbon (C)7-C8 is important for reducing the secretion of TNF-alpha. Interestingly, this effect did not show a simple dependence on concentration. The selected alkaloids showed little or no anti-COX activity. The results obtained from the present experiments may provide additional information useful in understanding the structure-to-activity relationship of the quaternary benzophenanthridine alkaloids. The anti-inflammatory potential and the cytotoxic effect are driven by the presence of a methylenedioxy ring attached at C7-C8 and C2-C3, respectively.

Návaznosti

MUNI/A/1205/2016, interní kód MU

Název: Příspěvek biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Akronym: mopopas)

Investor: Masarykova univerzita, Příspěvek biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

HOŠEK, Jan, Kristýna ŠEBRLOVÁ, Petra KAUCKÁ, Ondřej PEŠ a Eva TÁBORSKÁ. The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity. Acta Veterinaria Brno. Brno: Veterinární a farmaceutická univerzita Brno, 2017, roč. 86, č. 3, s. 223-230. ISSN 0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201786030223.

@article{1396414,
   author = {Hošek, Jan and Šebrlová, Kristýna and Kaucká, Petra and Peš, Ondřej and Táborská, Eva},
   article_location = {Brno},
   article_number = {3},
   doi = {http://dx.doi.org/10.2754/avb201786030223},
   keywords = {Cyclooxygenase; cytotoxicity; inflammation; TNF-alpha.},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria Brno},
   title = {The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity},
   volume = {86},
   year = {2017}
}

TY  - JOUR
ID  - 1396414
AU  - Hošek, Jan - Šebrlová, Kristýna - Kaucká, Petra - Peš, Ondřej - Táborská, Eva
PY  - 2017
TI  - The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity
JF  - Acta Veterinaria Brno
VL  - 86
IS  - 3
SP  - 223-230
EP  - 223-230
PB  - Veterinární a farmaceutická univerzita Brno
SN  - 00017213
KW  - Cyclooxygenase
KW  - cytotoxicity
KW  - inflammation
KW  - TNF-alpha.
N2  - Quaternary benzophenanthridine alkaloids are known to have a wide range of biological effects, including antimicrobial, antifungal, anti-inflammatory, and antitumour activities. However, only sanguinarine and chelerythrine have been studied intensively. The aim of this study was to evaluate the anti-inflammatory potential of the five minor quaternary benzophenanthridine alkaloids sanguilutine, sanguirubine, chelirubine, chelilutine, and macarpine in vitro and to compare them with more thoroughly studied sanguinarine and chelerythrine. Before making cell-based assays, the cytotoxicity of the alkaloids was evaluated. The anti-inflammatory potential of the chosen alkaloids was evaluated as for their ability to modulate the lipopolysaccharide-induced secretion of tumour necrosis factor alpha (TNF-alpha) in the macrophage-like cell line THP-1. The cyclooxygenase (COX)-1 and COX-2 inhibitory activities were also measured. The results indicate that the presence of a methylenedioxy ring attached at carbon (C)7-C8 is important for reducing the secretion of TNF-alpha. Interestingly, this effect did not show a simple dependence on concentration. The selected alkaloids showed little or no anti-COX activity. The results obtained from the present experiments may provide additional information useful in understanding the structure-to-activity relationship of the quaternary benzophenanthridine alkaloids. The anti-inflammatory potential and the cytotoxic effect are driven by the presence of a methylenedioxy ring attached at C7-C8 and C2-C3, respectively.
ER  -

HOŠEK, Jan, Kristýna ŠEBRLOVÁ, Petra KAUCKÁ, Ondřej PEŠ a Eva TÁBORSKÁ. The capability of minor quaternary benzophenanthridine alkaloids to inhibit TNF-alpha secretion and cyclooxygenase activity. \textit{Acta Veterinaria Brno}. Brno: Veterinární a farmaceutická univerzita Brno, 2017, roč.~86, č.~3, s.~223-230. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201786030223.

Podrobný výpis o publikaci