J 2018

Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs

MEADER, Ellie, Tomáš BÁRTA, Dario MELGUIZO-SANCHIS, Katarzyna TILGNER, David MONTANER et. al.

Základní údaje

Originální název

Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs

Autoři

MEADER, Ellie (826 Velká Británie a Severní Irsko), Tomáš BÁRTA (203 Česká republika, domácí), Dario MELGUIZO-SANCHIS (826 Velká Británie a Severní Irsko), Katarzyna TILGNER (826 Velká Británie a Severní Irsko), David MONTANER (724 Španělsko), Ashraf A. EL-HAROUNI (682 Saúdská Arábie), Lyle ARMSTRONG (826 Velká Británie a Severní Irsko) a Majlinda LAKO (826 Velká Británie a Severní Irsko, garant)

Vydání

Stem Cells, Hoboken, Wiley-Blackwell, 2018, 1066-5099

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 5.614

Kód RIV

RIV/00216224:14110/18:00102112

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000418942500007

Klíčová slova anglicky

Human embryonic stem cells; miRNAs; Epithelial-mesenchymal transition; Hematopoietic differentiation

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 10. 2. 2019 17:25, Soňa Böhmová

Anotace

V originále

Hematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression and are responsible for their failure to engraft. We compared the miRNA expression profile of hematopoietic progenitors derived from pluripotent stem cells to those derived from bone marrow and found that numerous miRNAs are too highly expressed in hematopoietic progenitors derived from pluripotent stem cells, and that most of these are inhibitors of epithelial-mesenchymal transition or metastasis (including miR-200b, miR-200c, miR-205, miR-148a, and miR-424). We hypothesize that the high expression of these factors, which promote an adherent phenotype, may be causing the defect in hematopoietic differentiation. However, inhibiting these miRNAs, individually or in multiplex, was insufficient to improve hematopoietic differentiation in vitro, suggesting that other miRNAs and/or genes may be involved in this process.