The intracellular concentration of homocysteine and related
thiols is negatively correlated to sperm quality after highly
effective method of sperm lysis

J 2017

The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ et. al.

Základní údaje

Originální název

The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis

Autoři

KRÁLÍKOVÁ, Michaela (203 Česká republika, garant, domácí), Igor CRHA (203 Česká republika, domácí), Martin HUSER (203 Česká republika, domácí), Jitka MELOUNOVÁ (203 Česká republika, domácí), Jana ŽÁKOVÁ (203 Česká republika, domácí), Milena MATEJOVIČOVÁ (203 Česká republika, domácí) a Pavel VENTRUBA (203 Česká republika, domácí)

Vydání

ANDROLOGIA, HOBOKEN, WILEY, 2017, 0303-4569

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.588

Kód RIV

RIV/00216224:14110/17:00095676

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1111/and.12702

UT WoS

000408626900011

Klíčová slova anglicky

glutathione; homocysteine; seminal plasma; spermatozoa; thiols

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 17:53, Soňa Böhmová

Anotace

V originále

The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.

Návaznosti

NS9661, projekt VaV

Název: Vliv metabolismu homocysteinu na poruchy plodnosti.

Investor: Ministerstvo zdravotnictví ČR, Vliv metabolismu homocysteinu na poruchy plodnosti

Citovat

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ, Milena MATEJOVIČOVÁ a Pavel VENTRUBA. The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis. ANDROLOGIA. HOBOKEN: WILEY, 2017, roč. 49, č. 7, s. 1-5. ISSN 0303-4569. Dostupné z: https://dx.doi.org/10.1111/and.12702.

@article{1396967,
   author = {Králíková, Michaela and Crha, Igor and Huser, Martin and Melounová, Jitka and Žáková, Jana and Matejovičová, Milena and Ventruba, Pavel},
   article_location = {HOBOKEN},
   article_number = {7},
   doi = {http://dx.doi.org/10.1111/and.12702},
   keywords = {glutathione; homocysteine; seminal plasma; spermatozoa; thiols},
   language = {eng},
   issn = {0303-4569},
   journal = {ANDROLOGIA},
   title = {The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis},
   volume = {49},
   year = {2017}
}

TY  - JOUR
ID  - 1396967
AU  - Králíková, Michaela - Crha, Igor - Huser, Martin - Melounová, Jitka - Žáková, Jana - Matejovičová, Milena - Ventruba, Pavel
PY  - 2017
TI  - The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis
JF  - ANDROLOGIA
VL  - 49
IS  - 7
SP  - 1-5
EP  - 1-5
PB  - WILEY
SN  - 03034569
KW  - glutathione
KW  - homocysteine
KW  - seminal plasma
KW  - spermatozoa
KW  - thiols
N2  - The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.
ER  -

KRÁLÍKOVÁ, Michaela, Igor CRHA, Martin HUSER, Jitka MELOUNOVÁ, Jana ŽÁKOVÁ, Milena MATEJOVIČOVÁ a Pavel VENTRUBA. The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis. \textit{ANDROLOGIA}. HOBOKEN: WILEY, 2017, roč.~49, č.~7, s.~1-5. ISSN~0303-4569. Dostupné z: https://dx.doi.org/10.1111/and.12702.

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