J 2017

New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection

ĎURČ, Pavol, František FORET, Eva POKOJOVÁ, Lukáš HOMOLA, Jana SKŘIČKOVÁ et. al.

Základní údaje

Originální název

New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection

Autoři

ĎURČ, Pavol (703 Slovensko, domácí), František FORET (203 Česká republika, domácí), Eva POKOJOVÁ (203 Česká republika), Lukáš HOMOLA (203 Česká republika), Jana SKŘIČKOVÁ (203 Česká republika, garant), Vladimír HEROUT (203 Česká republika), Milan DASTYCH (203 Česká republika), Hana VINOHRADSKÁ (203 Česká republika) a Petr KUBÁŇ (203 Česká republika, domácí)

Vydání

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.307

Kód RIV

RIV/00216224:14110/17:00095220

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000401408300002

Klíčová slova anglicky

Cystic fibrosis; Capillary electrophoresis; Sweat; Skin wipe; Ion ratio; Diagnosis

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 20. 3. 2018 16:46, Soňa Böhmová

Anotace

V originále

A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless conductometric detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 mu L deionized water. The skin-wipe sample is then extracted for 3 min into 400 mu L deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless conductometric detection using a double opposite end injection. A 20 mmol/L L-histidine/2-(N-'morpholino) ethanesulfonic acid and 2 mmol/L 18-crown-6 at pH 6 electrolyte can separate all the major ions in less than 7 min. Skin-wipe sample extracts from 30 study participants-ten adult patients with CF (25-50 years old), ten pediatric patients with CF (1-15 years old), and ten healthy control individuals (1-18 years old)-were obtained and analyzed. From the analyzed ions in all samples, a significant difference between chloride and potassium concentrations was found in the CF patients and healthy controls. We propose the use of the Cl-/K+ ratio rather than the absolute Cl- concentration and a cutoff value of 4 in skin-wipe sample extracts as an alternative to the conventional sweat chloride analysis. The proposed Cl-/K+ ion ratio proved to be a more reliable indicator, is independent of the patient's age, and allows better differentiation between non-CF individuals and CF patients having intermediate values on the Cl- sweat test.

Návaznosti

GA13-21919S, projekt VaV
Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.
Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu
LQ1601, projekt VaV
Název: CEITEC 2020 (Akronym: CEITEC2020)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020