Distinctive behaviour of live biopsy-derived carcinoma cells
unveiled using coherence-controlled holographic microscopy

J 2017

Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy

GÁL, Břetislav, Miroslav VESELÝ, Jana COLLAKOVA, Marta NEKULOVA, Veronika JUZOVA et. al.

Základní údaje

Originální název

Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy

Autoři

GÁL, Břetislav, Miroslav VESELÝ, Jana COLLAKOVA, Marta NEKULOVA, Veronika JUZOVA, Radim CHMELIK a Pavel VESELÝ

Vydání

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30206 Otorhinolaryngology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0183399

UT WoS

000408438600028

Klíčová slova anglicky

cancer; head; neck

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 13:05, Soňa Böhmová

Anotace

V originále

Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity should quickly indicate the suitability of chemotherapy and radiotherapy. No ex vivo chemosensitivity assessment developed thus far has become a part of general clinical practice. Therefore, we attempted to explore the new technique of coherence-controlled holographic microscopy to investigate the motility and growth of live cells from a head and neck squamous cell carcinoma biopsy. We expected to reveal behavioural patterns characteristic for malignant cells that can be used to imrove future predictive evaluation of chemotherapy. We managed to cultivate primary SACR2 carcinoma cells from head and neck squamous cell carcinoma biopsy verified through histopathology. The cells grew as a cohesive sheet of suspected carcinoma origin, and western blots showed positivity for the tumour marker p63 confirming cancerous origin. Unlike the roundish colonies of the established FaDu carcinoma cell line, the SACR2 cells formed irregularly shaped colonies, eliciting the impression of the collective invasion of carcinoma cells. Time-lapse recordings of the cohesive sheet activity revealed the rapid migration and high plasticity of these epithelial-like cells. Individual cells frequently abandoned the swiftly migrating crowd by moving aside and crawling faster. The increasing mass of fast migrating epithelial-like cells before and after mitosis confirmed the continuation of the cell cycle. In immunofluorescence, analogously shaped cells expressed the p63 tumour marker, considered proof of their origin from a carcinoma. These behavioural traits indicate the feasible identification of carcinoma cells in culture according to the proposed concept of the carcinoma cell dynamic phenotype. If further developed, this approach could later serve in a new functional online analysis of reactions of carcinoma cells to therapy. Such efforts conform to current trends in precision medicine.

Citovat

GÁL, Břetislav, Miroslav VESELÝ, Jana COLLAKOVA, Marta NEKULOVA, Veronika JUZOVA, Radim CHMELIK a Pavel VESELÝ. Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 8, s. 1-14. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0183399.

@article{1398294,
   author = {Gál, Břetislav and Veselý, Miroslav and Collakova, Jana and Nekulova, Marta and Juzova, Veronika and Chmelik, Radim and Veselý, Pavel},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0183399},
   keywords = {cancer; head; neck},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy},
   url = {http://dx.doi.org/10.1371/journal.pone.0183399},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1398294
AU  - Gál, Břetislav - Veselý, Miroslav - Collakova, Jana - Nekulova, Marta - Juzova, Veronika - Chmelik, Radim - Veselý, Pavel
PY  - 2017
TI  - Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy
JF  - Plos one
VL  - 12
IS  - 8
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19326203
KW  - cancer
KW  - head
KW  - neck
UR  - http://dx.doi.org/10.1371/journal.pone.0183399
L2  - http://dx.doi.org/10.1371/journal.pone.0183399
N2  - Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity should quickly indicate the suitability of chemotherapy and radiotherapy. No ex vivo chemosensitivity assessment developed thus far has become a part of general clinical practice. Therefore, we attempted to explore the new technique of coherence-controlled holographic microscopy to investigate the motility and growth of live cells from a head and neck squamous cell carcinoma biopsy. We expected to reveal behavioural patterns characteristic for malignant cells that can be used to imrove future predictive evaluation of chemotherapy. We managed to cultivate primary SACR2 carcinoma cells from head and neck squamous cell carcinoma biopsy verified through histopathology. The cells grew as a cohesive sheet of suspected carcinoma origin, and western blots showed positivity for the tumour marker p63 confirming cancerous origin. Unlike the roundish colonies of the established FaDu carcinoma cell line, the SACR2 cells formed irregularly shaped colonies, eliciting the impression of the collective invasion of carcinoma cells. Time-lapse recordings of the cohesive sheet activity revealed the rapid migration and high plasticity of these epithelial-like cells. Individual cells frequently abandoned the swiftly migrating crowd by moving aside and crawling faster. The increasing mass of fast migrating epithelial-like cells before and after mitosis confirmed the continuation of the cell cycle. In immunofluorescence, analogously shaped cells expressed the p63 tumour marker, considered proof of their origin from a carcinoma. These behavioural traits indicate the feasible identification of carcinoma cells in culture according to the proposed concept of the carcinoma cell dynamic phenotype. If further developed, this approach could later serve in a new functional online analysis of reactions of carcinoma cells to therapy. Such efforts conform to current trends in precision medicine.
ER  -

GÁL, Břetislav, Miroslav VESELÝ, Jana COLLAKOVA, Marta NEKULOVA, Veronika JUZOVA, Radim CHMELIK a Pavel VESELÝ. Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~8, s.~1-14. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0183399.

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