New modification of cultivation medium for isolation and growth
of intestinal sulfate-reducing bacteria

D 2017

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

KOVÁČ, Jozef a Ivan KUSHKEVYCH

Základní údaje

Originální název

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

Autoři

KOVÁČ, Jozef (703 Slovensko, domácí) a Ivan KUSHKEVYCH (804 Ukrajina, garant, domácí)

Vydání

Brno, Czech Republic, MendelNet 2017 Proceedings of 24th International PhD Students Conference, od s. 702-707, 6 s. 2017

Nakladatel

Faculty of AgriSciences, Mendel University in Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14310/17:00098646

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7509-529-9

UT WoS

000440194500125

Klíčová slova anglicky

sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 8. 2018 10:06, doc. Ivan Kushkevych, Ph.D.

Anotace

V originále

Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.

Návaznosti

MUNI/A/0906/2016, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 1 (Akronym: SVMIK)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 1, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KOVÁČ, Jozef a Ivan KUSHKEVYCH. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. Online. In MendelNet 2017 Proceedings of 24th International PhD Students Conference. Brno, Czech Republic: Faculty of AgriSciences, Mendel University in Brno, 2017, s. 702-707. ISBN 978-80-7509-529-9.

@inproceedings{1398314,
   author = {Kováč, Jozef and Kushkevych, Ivan},
   address = {Brno, Czech Republic},
   booktitle = {MendelNet 2017 Proceedings of 24th International PhD Students Conference},
   keywords = {sulfate-reducing bacteria; Desulfovibrio; cultivation media; modification of conditions; bacterial growth},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno, Czech Republic},
   isbn = {978-80-7509-529-9},
   pages = {702-707},
   publisher = {Faculty of AgriSciences, Mendel University in Brno},
   title = {New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria},
   url = {https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf},
   year = {2017}
}

TY  - JOUR
ID  - 1398314
AU  - Kováč, Jozef - Kushkevych, Ivan
PY  - 2017
TI  - New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria
PB  - Faculty of AgriSciences, Mendel University in Brno
CY  - Brno, Czech Republic
SN  - 9788075095299
KW  - sulfate-reducing bacteria
KW  - Desulfovibrio
KW  - cultivation media
KW  - modification of conditions
KW  - bacterial growth
UR  - https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
L2  - https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf
N2  - Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.
ER  -

KOVÁČ, Jozef a Ivan KUSHKEVYCH. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. Online. In \textit{MendelNet 2017 Proceedings of 24th International PhD Students Conference}. Brno, Czech Republic: Faculty of AgriSciences, Mendel University in Brno, 2017, s.~702-707. ISBN~978-80-7509-529-9.

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