The application of comet assay in monitoring of the
immunosuppression level in a patient with toxic epidermal
necrolysis: A case report

J 2017

The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ et. al.

Základní údaje

Originální název

The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report

Autoři

LIPOVÝ, Břetislav (203 Česká republika, garant, domácí), Milena MATEJOVIČOVÁ (203 Česká republika, domácí), H. ŘIHOVÁ (203 Česká republika), M. ŠTIKAROVSKÁ (203 Česká republika), Lucie NOVOTNÁ (203 Česká republika, domácí), Miroslava HLAVÁČOVÁ (703 Slovensko, domácí), Hana PAULOVÁ (203 Česká republika, domácí), I. SUCHÁNEK (203 Česká republika) a Pavel BRYCHTA (203 Česká republika)

Vydání

Burns Open, Amsterdam, Elsevier, 2017, 2468-9122

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/17:00098656

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.burnso.2017.05.007

Klíčová slova anglicky

Toxic epidermal necrolysis; Immunosuppression; Comet assay

Štítky

EL OK

Změněno: 19. 3. 2018 16:01, Soňa Böhmová

Anotace

V originále

Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation. The basic approach in the therapy of patients suffering from toxic epidermal necrolysis is the administration of immunosuppressive medications. Comet assay is a fast, sensitive and relatively easy method used to detect DNA damage levels. We present a case report of a woman with toxic epidermal necrolysis treated with cyclosporine A. During the therapy, comet assay was used to detect the level of DNA damage of circulating lymphocytes and it helped us to reveal qualitative abnormalities in these cells.

Citovat

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ, Miroslava HLAVÁČOVÁ, Hana PAULOVÁ, I. SUCHÁNEK a Pavel BRYCHTA. The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report. Burns Open. Amsterdam: Elsevier, 2017, roč. 1, č. 2, s. 80-83. ISSN 2468-9122. Dostupné z: https://dx.doi.org/10.1016/j.burnso.2017.05.007.

@article{1398340,
   author = {Lipový, Břetislav and Matejovičová, Milena and Řihová, H. and Štikarovská, M. and Novotná, Lucie and Hlaváčová, Miroslava and Paulová, Hana and Suchánek, I. and Brychta, Pavel},
   article_location = {Amsterdam},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.burnso.2017.05.007},
   keywords = {Toxic epidermal necrolysis; Immunosuppression; Comet assay},
   language = {eng},
   issn = {2468-9122},
   journal = {Burns Open},
   title = {The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report},
   volume = {1},
   year = {2017}
}

TY  - JOUR
ID  - 1398340
AU  - Lipový, Břetislav - Matejovičová, Milena - Řihová, H. - Štikarovská, M. - Novotná, Lucie - Hlaváčová, Miroslava - Paulová, Hana - Suchánek, I. - Brychta, Pavel
PY  - 2017
TI  - The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report
JF  - Burns Open
VL  - 1
IS  - 2
SP  - 80-83
EP  - 80-83
PB  - Elsevier
SN  - 24689122
KW  - Toxic epidermal necrolysis
KW  - Immunosuppression
KW  - Comet assay
N2  - Toxic epidermal necrolysis (TEN) represents the most serious affection within the SCARs group (Severe Cutaneous Adverse Reactions). It is an autoimmune disease manifested by extensive epidermal and mucosal exfoliation. The basic approach in the therapy of patients suffering from toxic epidermal necrolysis is the administration of immunosuppressive medications. Comet assay is a fast, sensitive and relatively easy method used to detect DNA damage levels. We present a case report of a woman with toxic epidermal necrolysis treated with cyclosporine A. During the therapy, comet assay was used to detect the level of DNA damage of circulating lymphocytes and it helped us to reveal qualitative abnormalities in these cells.
ER  -

LIPOVÝ, Břetislav, Milena MATEJOVIČOVÁ, H. ŘIHOVÁ, M. ŠTIKAROVSKÁ, Lucie NOVOTNÁ, Miroslava HLAVÁČOVÁ, Hana PAULOVÁ, I. SUCHÁNEK a Pavel BRYCHTA. The application of comet assay in monitoring of the immunosuppression level in a patient with toxic epidermal necrolysis: A case report. \textit{Burns Open}. Amsterdam: Elsevier, 2017, roč.~1, č.~2, s.~80-83. ISSN~2468-9122. Dostupné z: https://dx.doi.org/10.1016/j.burnso.2017.05.007.

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