Intact Cell Mass Spectrometry as a Quality Control Tool for
Revealing Minute Phenotypic Changes of Cultured Human Embryonic
Stem Cells

J 2018

Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells

VAŇHARA, Petr, Lukáš KUČERA, Lubomír PROKEŠ, Lucie JUREČKOVÁ, Eladia María PEÑA-MÉNDEZ et. al.

Základní údaje

Originální název

Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells

Autoři

VAŇHARA, Petr (203 Česká republika, domácí), Lukáš KUČERA (203 Česká republika, domácí), Lubomír PROKEŠ (203 Česká republika, domácí), Lucie JUREČKOVÁ (203 Česká republika, domácí), Eladia María PEÑA-MÉNDEZ (724 Španělsko), Josef HAVEL (203 Česká republika, domácí) a Aleš HAMPL (203 Česká republika, garant, domácí)

Vydání

Stem Cells Translational Medicine, Hoboken, Wiley, 2018, 2157-6564

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.962

Kód RIV

RIV/00216224:14110/18:00100768

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/sctm.17-0107

UT WoS

000419115400012

Klíčová slova anglicky

cell culture; differentiation; embryonic stem cells; technology; tissue engineering

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2019 10:47, Soňa Böhmová

Anotace

V originále

The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. Therefore, there is an ongoing need for robust, feasible, and sensitive methods of determining or confirming cell status and for revealing potential divergences from the optimal state. We modeled both intrinsic and extrinsic changes in human embryonic stem cell (hESC) states using different experimental strategies and addressed the changes in cell status by intact cell mass spectrometry fingerprinting. The analysis of spectral fingerprints by methods routinely used in analytical chemistry clearly distinguished the morphologically and biochemically similar populations of hESCs and provided a biomarker-independent tool for the quality control of cell culture

Návaznosti

GA15-11707S, projekt VaV

Název: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Grantová agentura ČR, Centrosomální abnormality u lidských pluripotentních kmenových buněk

LD15144, projekt VaV

Název: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Akronym: TissueENG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Buněčné a nebuněčné základy pro regeneraci kostí a zubů

MUNI/A/1369/2016, interní kód MU

Název: Zdroje pro tkáňové inženýrství 7 (Akronym: TissueENG 7)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 7, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

VAŇHARA, Petr, Lukáš KUČERA, Lubomír PROKEŠ, Lucie JUREČKOVÁ, Eladia María PEÑA-MÉNDEZ, Josef HAVEL a Aleš HAMPL. Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells. Stem Cells Translational Medicine. Hoboken: Wiley, 2018, roč. 7, č. 1, s. 109-114. ISSN 2157-6564. Dostupné z: https://dx.doi.org/10.1002/sctm.17-0107.

@article{1400984,
   author = {Vaňhara, Petr and Kučera, Lukáš and Prokeš, Lubomír and Jurečková, Lucie and PeñaandMéndez, Eladia María and Havel, Josef and Hampl, Aleš},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/sctm.17-0107},
   keywords = {cell culture; differentiation; embryonic stem cells; technology; tissue engineering},
   language = {eng},
   issn = {2157-6564},
   journal = {Stem Cells Translational Medicine},
   title = {Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/sctm.17-0107/abstract},
   volume = {7},
   year = {2018}
}

TY  - JOUR
ID  - 1400984
AU  - Vaňhara, Petr - Kučera, Lukáš - Prokeš, Lubomír - Jurečková, Lucie - Peña-Méndez, Eladia María - Havel, Josef - Hampl, Aleš
PY  - 2018
TI  - Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells
JF  - Stem Cells Translational Medicine
VL  - 7
IS  - 1
SP  - 109-114
EP  - 109-114
PB  - Wiley
SN  - 21576564
KW  - cell culture
KW  - differentiation
KW  - embryonic stem cells
KW  - technology
KW  - tissue engineering
UR  - http://onlinelibrary.wiley.com/doi/10.1002/sctm.17-0107/abstract
N2  - The stability of in vitro cell cultures is an important issue for any clinical, bio-industrial, or pharmacological use. Embryonic stem cells are pluripotent; consequently, they possess the ability to differentiate into all three germ layers and are inherently prone to respond to differentiation stimuli. However, long-term culture inevitably yields clones that are best adapted to the culture conditions, passaging regimes, or differentiation sensitivity. This cellular plasticity is a major obstacle in the development of bio-industrial or clinical-grade cultures. At present, the quality control of cell cultures is limited by the lack of reliable (epi)genetic or molecular markers or by the focus on a particular type of instability such as karyotype abnormalities or adverse phenotypic traits. Therefore, there is an ongoing need for robust, feasible, and sensitive methods of determining or confirming cell status and for revealing potential divergences from the optimal state. We modeled both intrinsic and extrinsic changes in human embryonic stem cell (hESC) states using different experimental strategies and addressed the changes in cell status by intact cell mass spectrometry fingerprinting. The analysis of spectral fingerprints by methods routinely used in analytical chemistry clearly distinguished the morphologically and biochemically similar populations of hESCs and provided a biomarker-independent tool for the quality control of cell culture
ER  -

VAŇHARA, Petr, Lukáš KUČERA, Lubomír PROKEŠ, Lucie JUREČKOVÁ, Eladia María PEÑA-MÉNDEZ, Josef HAVEL a Aleš HAMPL. Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells. \textit{Stem Cells Translational Medicine}. Hoboken: Wiley, 2018, roč.~7, č.~1, s.~109-114. ISSN~2157-6564. Dostupné z: https://dx.doi.org/10.1002/sctm.17-0107.

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