A preliminary study of endocannabinoid system regulation in
psychosis: Distinct alterations of CNR1 promoter DNA
methylation in patients with schizophrenia

J 2017

A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI et. al.

Základní údaje

Originální název

A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia

Autoři

ADDARIO, D. (380 Itálie), Vincenzo MICALE (380 Itálie, domácí), M. DI BARTOLOMEO (380 Itálie), Tibor ŠTARK (703 Slovensko, garant, domácí), M. PUCCI (380 Itálie), Alexandra ŠULCOVÁ (203 Česká republika, domácí), M. PALAZZO (380 Itálie), Zuzana BABINSKÁ (703 Slovensko, domácí), L. CREMASCHI (380 Itálie), F. DRAGO (380 Itálie), C.A. ALTAMURA (380 Itálie), M. MACCARRONE (380 Itálie) a B. DELL'OSSO (380 Itálie)

Vydání

Schizophrenia Research, AMSTERDAM, ELSEVIER SCIENCE BV, 2017, 0920-9964

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30215 Psychiatry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.958

Kód RIV

RIV/00216224:14110/17:00099040

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.schres.2017.01.022

UT WoS

000415217800021

Klíčová slova anglicky

Endocannabinoid system (ECS); Cannabinoid receptor type-1; DNA methylation; Schizophrenia; Methylazoxymethanol (MAM) rat model

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 18:10, Soňa Böhmová

Anotace

V originále

Compelling evidence supports the involvement of the endocannabinoid system(ECS) in psychosis vulnerability. We here evaluated the transcriptional regulation of ECS components in human peripheral blood mononuclear cells (PBMCs) obtained from subjects suffering from bipolar disorder, major depressive disorder and schizophrenia, focusing in particular on the effects of DNA methylation. We observed selective alterations of DNA methylation at the promoter of CNR1, the gene coding for the type-1 cannabinoid receptor, in schizophrenic patients (N = 25) with no changes in any other disorder. We confirmed the regulation of CNR1 in a well-validated animal model of schizophrenia, induced by prenatal methylazoxymethanol (MAM) acetate exposure (N = 7 per group) where we found, in the prefrontal cortex, a significant increase in CNR1 expression and a consistent reduction in DNA methylation at specific CpG sites of gene promoter. Overall, our findings suggest a selective dysregulation of ECS in psychosis, and highlight the evaluation of CNR1 DNA methylation levels in PBMCs as a potential biomarker for schizophrenia. (C) 2017 Elsevier B.V. All rights reserved.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1063/2016, interní kód MU

Název: Experimentální a translační farmakologický výzkum a vývoj

Investor: Masarykova univerzita, Experimentální a translační farmakologický výzkum a vývoj, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI, Alexandra ŠULCOVÁ, M. PALAZZO, Zuzana BABINSKÁ, L. CREMASCHI, F. DRAGO, C.A. ALTAMURA, M. MACCARRONE a B. DELL'OSSO. A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia. Schizophrenia Research. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, roč. 188, OCT 2017, s. 132-140. ISSN 0920-9964. Dostupné z: https://dx.doi.org/10.1016/j.schres.2017.01.022.

@article{1400993,
   author = {Addario, D. and Micale, Vincenzo and Di Bartolomeo, M. and Štark, Tibor and Pucci, M. and Šulcová, Alexandra and Palazzo, M. and Babinská, Zuzana and Cremaschi, L. and Drago, F. and Altamura, C.A. and Maccarrone, M. and Dell'Osso, B.},
   article_location = {AMSTERDAM},
   article_number = {OCT 2017},
   doi = {http://dx.doi.org/10.1016/j.schres.2017.01.022},
   keywords = {Endocannabinoid system (ECS); Cannabinoid receptor type-1; DNA methylation; Schizophrenia; Methylazoxymethanol (MAM) rat model},
   language = {eng},
   issn = {0920-9964},
   journal = {Schizophrenia Research},
   title = {A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia},
   volume = {188},
   year = {2017}
}

TY  - JOUR
ID  - 1400993
AU  - Addario, D. - Micale, Vincenzo - Di Bartolomeo, M. - Štark, Tibor - Pucci, M. - Šulcová, Alexandra - Palazzo, M. - Babinská, Zuzana - Cremaschi, L. - Drago, F. - Altamura, C.A. - Maccarrone, M. - Dell'Osso, B.
PY  - 2017
TI  - A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia
JF  - Schizophrenia Research
VL  - 188
IS  - OCT 2017
SP  - 132-140
EP  - 132-140
PB  - ELSEVIER SCIENCE BV
SN  - 09209964
KW  - Endocannabinoid system (ECS)
KW  - Cannabinoid receptor type-1
KW  - DNA methylation
KW  - Schizophrenia
KW  - Methylazoxymethanol (MAM) rat model
N2  - Compelling evidence supports the involvement of the endocannabinoid system(ECS) in psychosis vulnerability. We here evaluated the transcriptional regulation of ECS components in human peripheral blood mononuclear cells (PBMCs) obtained from subjects suffering from bipolar disorder, major depressive disorder and schizophrenia, focusing in particular on the effects of DNA methylation. We observed selective alterations of DNA methylation at the promoter of CNR1, the gene coding for the type-1 cannabinoid receptor, in schizophrenic patients (N = 25) with no changes in any other disorder. We confirmed the regulation of CNR1 in a well-validated animal model of schizophrenia, induced by prenatal methylazoxymethanol (MAM) acetate exposure (N = 7 per group) where we found, in the prefrontal cortex, a significant increase in CNR1 expression and a consistent reduction in DNA methylation at specific CpG sites of gene promoter. Overall, our findings suggest a selective dysregulation of ECS in psychosis, and highlight the evaluation of CNR1 DNA methylation levels in PBMCs as a potential biomarker for schizophrenia. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

ADDARIO, D., Vincenzo MICALE, M. DI BARTOLOMEO, Tibor ŠTARK, M. PUCCI, Alexandra ŠULCOVÁ, M. PALAZZO, Zuzana BABINSKÁ, L. CREMASCHI, F. DRAGO, C.A. ALTAMURA, M. MACCARRONE a B. DELL'OSSO. A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia. \textit{Schizophrenia Research}. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, roč.~188, OCT 2017, s.~132-140. ISSN~0920-9964. Dostupné z: https://dx.doi.org/10.1016/j.schres.2017.01.022.

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