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@inbook{1401759,
author = {Ešner, Milan and Meyenhofer, Felix and Bickle, Marc},
address = {New York},
booktitle = {High Content Screening},
doi = {http://dx.doi.org/10.1007/978-1-4939-7357-6_10},
editor = {Milan Ešner},
keywords = {Drug development; Imaging; Image analysis; Live cell; Kinetic; Environmental control},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-4939-7355-2},
pages = {149-164},
publisher = {Humana Press},
title = {Live-Cell High Content Screening in Drug Development},
url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-7357-6_10},
year = {2018}
}
TY - CHAP
ID - 1401759
AU - Ešner, Milan - Meyenhofer, Felix - Bickle, Marc
PY - 2018
TI - Live-Cell High Content Screening in Drug Development
VL - Methods in Molecular Biology, Vol. 1683
PB - Humana Press
CY - New York
SN - 9781493973552
KW - Drug development
KW - Imaging
KW - Image analysis
KW - Live cell
KW - Kinetic
KW - Environmental control
UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-7357-6_10
N2 - In the past decade, automated microscopy has become an important tool for the drug discovery and development process. The establishment of imaging modalities as screening tools depended on technological breakthroughs in the domain of automated microscopy and automated image analysis. These types of assays are often referred to as high content screening or high content analysis (HCS/HCA). The driving force to adopt imaging for drug development is the quantity and quality of cellular information that can be collected and the enhanced physiological relevance of cellular screening compared to biochemical screening. Most imaging in drug development is performed on fixed cells as this allows uncoupling the preparation of the cells from the acquisition of the images. Live-cell imaging is technically challenging, but is very useful for many aspects of the drug development pipeline such as kinetic studies of compound mode of action or to analyze the motion of cellular components. Most vendors of HCS microscopy systems offer the option of environmental chambers and onboard pipetting on their platforms. This reflects the wish and desire of many customers to have the ability to perform live-cell assays on their HCS automated microscopes. This book chapter summarizes the challenges and advantages of live-cell imaging in drug discovery. Examples of applications are presented and the motivation to perform these assays in kinetic mode is discussed.
ER -
EŠNER, Milan, Felix MEYENHOFER a Marc BICKLE. Live-Cell High Content Screening in Drug Development. In Milan Ešner. \textit{High Content Screening}. New York: Humana Press, 2018, s.~149-164. Methods in Molecular Biology, Vol. 1683. ISBN~978-1-4939-7355-2. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-7357-6\_{}10.
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