Reprogramming of Adult Peripheral Blood Cells into Human
Induced Pluripotent Stem Cells as a Safe and Accessible Source
of Endothelial Cells.

J 2018

Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ et. al.

Základní údaje

Originální název

Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.

Autoři

ŠIMARA, Pavel (203 Česká republika, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí), Daniela ŘEHÁKOVÁ (203 Česká republika, domácí), Šimon FARKAŠ (703 Slovensko, domácí), Barbara ŠALINGOVÁ (703 Slovensko, domácí), Kateřina KUTÁLKOVÁ (203 Česká republika, domácí), Eva VAVREČKOVÁ (203 Česká republika, domácí), Pavel MATULA (203 Česká republika, domácí), Petr MATULA (203 Česká republika, domácí), Lenka VEVERKOVÁ (203 Česká republika) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

Stem Cells and Development, Mary Ann Liebert, Inc. publishers, 2018, 1547-3287

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.147

Kód RIV

RIV/00216224:14330/18:00100772

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1089/scd.2017.0132

UT WoS

000417686200001

Klíčová slova anglicky

induced pluripotent stem cells; endothelial differentiation; peripheral blood mononuclear cells

Štítky

cbia-web

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2019 16:10, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

New approaches in regenerative medicine and vasculogenesis have generated a demand for sufficient numbers of human endothelial cells (ECs). ECs and their progenitors reside on the interior surface of blood and lymphatic vessels or circulate in peripheral blood; however, their numbers are limited, and they are difficult to expand after isolation. Recent advances in human induced pluripotent stem cell (hiPSC) research have opened possible avenues to generate unlimited numbers of ECs from easily accessible cell sources, such as the peripheral blood. In this study, we reprogrammed peripheral blood mononuclear cells, human umbilical vein endothelial cells (HUVECs), and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. The phenotype profiles, functionality, and genome stability of all hiPSC-derived ECs were assessed and compared with HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts, as shown by the expression of the endothelial surface markers CD31 and CD144 and the results of the functional analysis. Higher expression of endothelial progenitor markers CD34 and kinase insert domain receptor (KDR) was measured in hiPSC-derived ECs. An analysis of phosphorylated histone H2AX (gamma-H2AX) foci revealed that an increased number of DNA double-strand breaks upon reprogramming into pluripotent cells. However, differentiation into ECs restored a normal number of gamma-H2AX foci. Our hiPSCs retained a normal karyotype, with the exception of the HSVEC-derived hiPSC line, which displayed mosaicism due to a gain of chromosome 1. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage. hiPSC-derived ECs are fully functional and comparable to natural ECs. The protocol is eligible for clinical applications in regenerative medicine, if the genomic stability of the pluripotent cell stage is closely monitored.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA, Lenka VEVERKOVÁ a Irena KRONTORÁD KOUTNÁ. Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells. Stem Cells and Development. Mary Ann Liebert, Inc. publishers, 2018, roč. 27, č. 1, s. 10-22. ISSN 1547-3287. Dostupné z: https://dx.doi.org/10.1089/scd.2017.0132.

@article{1401784,
   author = {Šimara, Pavel and Tesařová, Lenka and Řeháková, Daniela and Farkaš, Šimon and Šalingová, Barbara and Kutálková, Kateřina and Vavrečková, Eva and Matula, Pavel and Matula, Petr and Veverková, Lenka and Krontorád Koutná, Irena},
   article_number = {1},
   doi = {http://dx.doi.org/10.1089/scd.2017.0132},
   keywords = {induced pluripotent stem cells; endothelial differentiation; peripheral blood mononuclear cells},
   language = {eng},
   issn = {1547-3287},
   journal = {Stem Cells and Development},
   title = {Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.},
   url = {http://online.liebertpub.com/doi/10.1089/scd.2017.0132},
   volume = {27},
   year = {2018}
}

TY  - JOUR
ID  - 1401784
AU  - Šimara, Pavel - Tesařová, Lenka - Řeháková, Daniela - Farkaš, Šimon - Šalingová, Barbara - Kutálková, Kateřina - Vavrečková, Eva - Matula, Pavel - Matula, Petr - Veverková, Lenka - Krontorád Koutná, Irena
PY  - 2018
TI  - Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells.
JF  - Stem Cells and Development
VL  - 27
IS  - 1
SP  - 10-22
EP  - 10-22
PB  - Mary Ann Liebert, Inc. publishers
SN  - 15473287
KW  - induced pluripotent stem cells
KW  - endothelial differentiation
KW  - peripheral blood mononuclear cells
UR  - http://online.liebertpub.com/doi/10.1089/scd.2017.0132
L2  - http://online.liebertpub.com/doi/10.1089/scd.2017.0132
N2  - New approaches in regenerative medicine and vasculogenesis have generated a demand for sufficient numbers of human endothelial cells (ECs). ECs and their progenitors reside on the interior surface of blood and lymphatic vessels or circulate in peripheral blood; however, their numbers are limited, and they are difficult to expand after isolation. Recent advances in human induced pluripotent stem cell (hiPSC) research have opened possible avenues to generate unlimited numbers of ECs from easily accessible cell sources, such as the peripheral blood. In this study, we reprogrammed peripheral blood mononuclear cells, human umbilical vein endothelial cells (HUVECs), and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. The phenotype profiles, functionality, and genome stability of all hiPSC-derived ECs were assessed and compared with HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts, as shown by the expression of the endothelial surface markers CD31 and CD144 and the results of the functional analysis. Higher expression of endothelial progenitor markers CD34 and kinase insert domain receptor (KDR) was measured in hiPSC-derived ECs. An analysis of phosphorylated histone H2AX (gamma-H2AX) foci revealed that an increased number of DNA double-strand breaks upon reprogramming into pluripotent cells. However, differentiation into ECs restored a normal number of gamma-H2AX foci. Our hiPSCs retained a normal karyotype, with the exception of the HSVEC-derived hiPSC line, which displayed mosaicism due to a gain of chromosome 1. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage. hiPSC-derived ECs are fully functional and comparable to natural ECs. The protocol is eligible for clinical applications in regenerative medicine, if the genomic stability of the pluripotent cell stage is closely monitored.
ER  -

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA, Lenka VEVERKOVÁ a Irena KRONTORÁD KOUTNÁ. Reprogramming of Adult Peripheral Blood Cells into Human Induced Pluripotent Stem Cells as a Safe and Accessible Source of Endothelial Cells. \textit{Stem Cells and Development}. Mary Ann Liebert, Inc. publishers, 2018, roč.~27, č.~1, s.~10-22. ISSN~1547-3287. Dostupné z: https://dx.doi.org/10.1089/scd.2017.0132.

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