2018
Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
KNAPP, Michal, Pavel DOBEŠ, Michal ŘEŘICHA a Pavel HYRŠLZákladní údaje
Originální název
Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Název česky
Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods
Autoři
KNAPP, Michal (203 Česká republika), Pavel DOBEŠ (203 Česká republika, garant, domácí), Michal ŘEŘICHA (203 Česká republika) a Pavel HYRŠL (203 Česká republika, domácí)
Vydání
European Journal of Entomology, AV ČR, Institute of Entomology of the Czech Academy of Sciences, 2018, 1802-8829
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30102 Immunology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 0.965
Kód RIV
RIV/00216224:14310/18:00102177
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000434150700001
Klíčová slova česky
Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invazivní druh; hemolymfa; antimikrobiální aktivita; koncentrace hemocytů; koncenttrace proteinů; reflexní krvácení; metoda vzorkování
Klíčová slova anglicky
Coleoptera; Coccinellidae; Harmonia axyridis; Coccinella septempunctata; Ceratomegilla undecimnotata; invasive species; haemolymph; antimicrobial activity; haemocyte concentration; protein concentration; reflex bleeding; sampling technique
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 14. 10. 2018 15:24, Mgr. Pavel Dobeš, Ph.D.
V originále
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.
Česky
Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of refl ex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the same concentration of haemocytes and total protein, and level of antimicrobial activity against Escherichia coli as in samples of haemolymph collected from within the body cavity. Therefore, reflex blood is a promising source of haemolymph for use in many physiological and immunological studies. It is the best way of collecting haemolymph if one wants to avoid challenging the immune system and concurrently also enables one to repeatedly collect haemolymph from one individual without injuring it, which would initiate repair mechanisms. The interspecific comparison indicates signifi cant differences among the species studied in three characteristics measured. Interestingly, the native species Ceratomegilla undecimnotata has nearly as effective immune system as the invasive Harmonia axyridis based on the level of antibacterial activity against Escherichia coli.