Butyrate and docosahexaenoic acid interact in alterations of
specific lipid classes in differentiating colon cancer cells.

J 2018

Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.

TYLICHOVÁ, Zuzana, Josef SLAVÍK, Miroslav CIGANEK, Petra OVESNÁ, Pavel KRČMÁŘ et. al.

Základní údaje

Originální název

Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.

Autoři

TYLICHOVÁ, Zuzana (203 Česká republika, domácí), Josef SLAVÍK (203 Česká republika), Miroslav CIGANEK (203 Česká republika, domácí), Petra OVESNÁ (203 Česká republika, domácí), Pavel KRČMÁŘ (203 Česká republika), Nicol STRAKOVÁ (203 Česká republika, domácí), Miroslav MACHALA (203 Česká republika, domácí), Alois KOZUBÍK (203 Česká republika, domácí), Jiřina HOFMANOVÁ (203 Česká republika, domácí) a Jan VONDRÁČEK (203 Česká republika, garant, domácí)

Vydání

JOURNAL OF CELLULAR BIOCHEMISTRY, Spojené státy americké, Wiley, 2018, 0730-2312

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.448

Kód RIV

RIV/00216224:14310/18:00102187

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/jcb.26641

UT WoS

000430667200041

Klíčová slova anglicky

butyrate; ceramides; colon cancer; docosahexaenoic acid; lipid analyses; phospholipids

Štítky

14119612, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 10:53, Mgr. Michal Petr

Anotace

V originále

Docosahexaenoic acid (DHA) and sodium butyrate (NaBt) exhibit a number of interactive effects on colon cancer cell growth, differentiation, or apoptosis; however, the molecular mechanisms responsible for these interactions and their impact on cellular lipidome are still not fully clear. Here, we show that both dietary agents together induce dynamic alterations of lipid metabolism, specific cellular lipid classes, and fatty acid composition. In HT-29 cell line, a model of differentiating colon carcinoma cells, NaBt supported incorporation of free DHA into non-polar lipids and their accumulation in cytoplasmic lipid droplets. DHA itself was not incorporated into sphingolipids; however, it significantly altered representation of individual ceramide (Cer) classes, in particular in combination with NaBt (DHA/NaBt). We observed altered expression of enzymes involved in Cer metabolism in cells treated with NaBt or DHA/NaBt, and exogenous Cer 16:0 was found to promote induction of apoptosis in differentiating HT-29 cells. NaBt, together with DHA, increased n-3 fatty acid synthesis and attenuated metabolism of monounsaturated fatty acids. Finally, DHA and/or NaBt altered expression of proteins involved in synthesis of fatty acids, including elongase 5, stearoyl CoA desaturase 1, or fatty acid synthase, with NaBt increasing expression of caveolin-1 and CD36 transporter, which may further promote DHA incorporation and its impact on cellular lipidome. In conclusion, our results indicate that interactions of DHA and NaBt exert complex changes in cellular lipidome, which may contribute to the alterations of colon cancer cell differentiation/apoptotic responses. The present data extend our knowledge about the nature of interactive effects of dietary fatty acids.

Citovat

TYLICHOVÁ, Zuzana, Josef SLAVÍK, Miroslav CIGANEK, Petra OVESNÁ, Pavel KRČMÁŘ, Nicol STRAKOVÁ, Miroslav MACHALA, Alois KOZUBÍK, Jiřina HOFMANOVÁ a Jan VONDRÁČEK. Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells. JOURNAL OF CELLULAR BIOCHEMISTRY. Spojené státy americké: Wiley, 2018, roč. 119, č. 6, s. 4664-4679. ISSN 0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.26641.

@article{1403063,
   author = {Tylichová, Zuzana and Slavík, Josef and Ciganek, Miroslav and Ovesná, Petra and Krčmář, Pavel and Straková, Nicol and Machala, Miroslav and Kozubík, Alois and Hofmanová, Jiřina and Vondráček, Jan},
   article_location = {Spojené státy americké},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/jcb.26641},
   keywords = {butyrate; ceramides; colon cancer; docosahexaenoic acid; lipid analyses; phospholipids},
   language = {eng},
   issn = {0730-2312},
   journal = {JOURNAL OF CELLULAR BIOCHEMISTRY},
   title = {Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.},
   url = {http://dx.doi.org/10.1002/jcb.26641},
   volume = {119},
   year = {2018}
}

TY  - JOUR
ID  - 1403063
AU  - Tylichová, Zuzana - Slavík, Josef - Ciganek, Miroslav - Ovesná, Petra - Krčmář, Pavel - Straková, Nicol - Machala, Miroslav - Kozubík, Alois - Hofmanová, Jiřina - Vondráček, Jan
PY  - 2018
TI  - Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells.
JF  - JOURNAL OF CELLULAR BIOCHEMISTRY
VL  - 119
IS  - 6
SP  - 4664-4679
EP  - 4664-4679
PB  - Wiley
SN  - 07302312
KW  - butyrate
KW  - ceramides
KW  - colon cancer
KW  - docosahexaenoic acid
KW  - lipid analyses
KW  - phospholipids
UR  - http://dx.doi.org/10.1002/jcb.26641
N2  - Docosahexaenoic acid (DHA) and sodium butyrate (NaBt) exhibit a number of interactive effects on colon cancer cell growth, differentiation, or apoptosis; however, the molecular mechanisms responsible for these interactions and their impact on cellular lipidome are still not fully clear. Here, we show that both dietary agents together induce dynamic alterations of lipid metabolism, specific cellular lipid classes, and fatty acid composition. In HT-29 cell line, a model of differentiating colon carcinoma cells, NaBt supported incorporation of free DHA into non-polar lipids and their accumulation in cytoplasmic lipid droplets. DHA itself was not incorporated into sphingolipids; however, it significantly altered representation of individual ceramide (Cer) classes, in particular in combination with NaBt (DHA/NaBt). We observed altered expression of enzymes involved in Cer metabolism in cells treated with NaBt or DHA/NaBt, and exogenous Cer 16:0 was found to promote induction of apoptosis in differentiating HT-29 cells. NaBt, together with DHA, increased n-3 fatty acid synthesis and attenuated metabolism of monounsaturated fatty acids. Finally, DHA and/or NaBt altered expression of proteins involved in synthesis of fatty acids, including elongase 5, stearoyl CoA desaturase 1, or fatty acid synthase, with NaBt increasing expression of caveolin-1 and CD36 transporter, which may further promote DHA incorporation and its impact on cellular lipidome. In conclusion, our results indicate that interactions of DHA and NaBt exert complex changes in cellular lipidome, which may contribute to the alterations of colon cancer cell differentiation/apoptotic responses. The present data extend our knowledge about the nature of interactive effects of dietary fatty acids.
ER  -

TYLICHOVÁ, Zuzana, Josef SLAVÍK, Miroslav CIGANEK, Petra OVESNÁ, Pavel KRČMÁŘ, Nicol STRAKOVÁ, Miroslav MACHALA, Alois KOZUBÍK, Jiřina HOFMANOVÁ a Jan VONDRÁČEK. Butyrate and docosahexaenoic acid interact in alterations of specific lipid classes in differentiating colon cancer cells. \textit{JOURNAL OF CELLULAR BIOCHEMISTRY}. Spojené státy americké: Wiley, 2018, roč.~119, č.~6, s.~4664-4679. ISSN~0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.26641.

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