Expression Profile of Genes Regulating Steroid Biosynthesis and
Metabolism in Human Ovarian Granulosa Cells-A Primary Culture
Approach

J 2017

Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach

KRANC, W., M. BRAZERT, K. OZEGOWSKA, M. J. NAWROCKI, J. BUDNA et. al.

Základní údaje

Originální název

Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach

Autoři

KRANC, W., M. BRAZERT, K. OZEGOWSKA, M. J. NAWROCKI, J. BUDNA, P. CELICHOWSKI, M. DYSZKIEWICZ-KONWINSKA, M. JANKOWSKI, Michal JEŠETA (203 Česká republika, domácí), L. PAWELCZYK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY (garant)

Vydání

International Journal of Molecular Sciences, Basel, MDPI, 2017, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.687

Kód RIV

RIV/00216224:14110/17:00120335

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/ijms18122673

UT WoS

000418896700176

Klíčová slova anglicky

human; granulosa cells; in vitro culture (IVC); steroid biosynthesis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 5. 2022 10:19, Mgr. Tereza Miškechová

Anotace

V originále

Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy. Understanding of the exact molecular mechanisms underlying those processes could not only provide us with deep insight into the regulation of the reproductive cycle, but also create new clinical advantages in detection and treatment of various diseases associated with sex hormone abnormalities. We have used the microarray approach validated by RT-qPCR, to analyze the patterns of gene expression in primary cultures of human granulosa cells at days 1, 7, 15, and 30 of said cultures. We have especially focused on genes belonging to ontology groups associated with steroid biosynthesis and metabolism, namely "Regulation of steroid biosynthesis process" and "Regulation of steroid metabolic process". Eleven genes have been chosen, as they exhibited major change under a culture condition. Out of those, ten genes, namely STAR, SCAP, POR, SREBF1, GFI1, SEC14L2, STARD4, INSIG1, DHCR7, and IL1B, belong to both groups. Patterns of expression of those genes were analyzed, along with brief description of their functions. That analysis helped us achieve a better understanding of the exact molecular processes underlying steroid biosynthesis and metabolism in human granulosa cells.

Citovat

KRANC, W., M. BRAZERT, K. OZEGOWSKA, M. J. NAWROCKI, J. BUDNA, P. CELICHOWSKI, M. DYSZKIEWICZ-KONWINSKA, M. JANKOWSKI, Michal JEŠETA, L. PAWELCZYK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY. Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach. International Journal of Molecular Sciences. Basel: MDPI, 2017, roč. 18, č. 12, s. 1-14. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms18122673.

@article{1403082,
   author = {Kranc, W. and Brazert, M. and Ozegowska, K. and Nawrocki, M. J. and Budna, J. and Celichowski, P. and DyszkiewiczandKonwinska, M. and Jankowski, M. and Ješeta, Michal and Pawelczyk, L. and Bruska, M. and Nowicki, M. and Zabel, M. and Kempisty, B.},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/ijms18122673},
   keywords = {human; granulosa cells; in vitro culture (IVC); steroid biosynthesis},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach},
   url = {http://dx.doi.org/10.3390/ijms18122673},
   volume = {18},
   year = {2017}
}

TY  - JOUR
ID  - 1403082
AU  - Kranc, W. - Brazert, M. - Ozegowska, K. - Nawrocki, M. J. - Budna, J. - Celichowski, P. - Dyszkiewicz-Konwinska, M. - Jankowski, M. - Ješeta, Michal - Pawelczyk, L. - Bruska, M. - Nowicki, M. - Zabel, M. - Kempisty, B.
PY  - 2017
TI  - Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach
JF  - International Journal of Molecular Sciences
VL  - 18
IS  - 12
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 14220067
KW  - human
KW  - granulosa cells
KW  - in vitro culture (IVC)
KW  - steroid biosynthesis
UR  - http://dx.doi.org/10.3390/ijms18122673
L2  - http://dx.doi.org/10.3390/ijms18122673
N2  - Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy. Understanding of the exact molecular mechanisms underlying those processes could not only provide us with deep insight into the regulation of the reproductive cycle, but also create new clinical advantages in detection and treatment of various diseases associated with sex hormone abnormalities. We have used the microarray approach validated by RT-qPCR, to analyze the patterns of gene expression in primary cultures of human granulosa cells at days 1, 7, 15, and 30 of said cultures. We have especially focused on genes belonging to ontology groups associated with steroid biosynthesis and metabolism, namely "Regulation of steroid biosynthesis process" and "Regulation of steroid metabolic process". Eleven genes have been chosen, as they exhibited major change under a culture condition. Out of those, ten genes, namely STAR, SCAP, POR, SREBF1, GFI1, SEC14L2, STARD4, INSIG1, DHCR7, and IL1B, belong to both groups. Patterns of expression of those genes were analyzed, along with brief description of their functions. That analysis helped us achieve a better understanding of the exact molecular processes underlying steroid biosynthesis and metabolism in human granulosa cells.
ER  -

KRANC, W., M. BRAZERT, K. OZEGOWSKA, M. J. NAWROCKI, J. BUDNA, P. CELICHOWSKI, M. DYSZKIEWICZ-KONWINSKA, M. JANKOWSKI, Michal JEŠETA, L. PAWELCZYK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY. Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells-A Primary Culture Approach. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2017, roč.~18, č.~12, s.~1-14. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms18122673.

Podrobný výpis o publikaci