Další formáty:
BibTeX
LaTeX
RIS
@proceedings{1404306,
author = {Kyseľ, Peter and Kostlánová, Nikola and Houser, Josef and Dubská, Eva and Šulák, Ondřej and Wimmerová, Michaela},
booktitle = {XVIII. setkání biochemiků a molekulárních biologů},
keywords = {Ralstonia; phytopathogen; lectin; trimer; protein purification; SDS-PAGE; protein stability; nano differential scanning fluorimetry; isothermal titration calorimetry; analytical ultracentrifugation; protein crystallization; X-ray diffraction},
language = {eng},
isbn = {978-80-210-8765-1},
title = {Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum},
year = {2017}
}
TY - CONF
ID - 1404306
AU - Kyseľ, Peter - Kostlánová, Nikola - Houser, Josef - Dubská, Eva - Šulák, Ondřej - Wimmerová, Michaela
PY - 2017
TI - Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum
SN - 9788021087651
KW - Ralstonia
KW - phytopathogen
KW - lectin
KW - trimer
KW - protein purification
KW - SDS-PAGE
KW - protein stability
KW - nano differential scanning fluorimetry
KW - isothermal titration calorimetry
KW - analytical ultracentrifugation
KW - protein crystallization
KW - X-ray diffraction
N2 - Lectin RS20L from phytopathogen Ralstonia solanacearum showed a low solubility without a sugar ligand. A selected panel of additives was tested using the nano differential scanning fluorimetry (nanoDSF). The manganese chloride together with higher pH (8.1) has shown to dramaticaly increase the stability important for crystallization.of RS20L protein. Crystallization screening provided crystals of RS20L in conditions of 0.1 M Hepes pH=7.5 with 10% (v/v) polyethylene glycol of different molecular weight. Increasing the temperature dramaticaly improved the reproducibility of crystallization.
ER -
KYSEĽ, Peter, Nikola KOSTLÁNOVÁ, Josef HOUSER, Eva DUBSKÁ, Ondřej ŠULÁK a Michaela WIMMEROVÁ. Crystallization and stability studies of trimeric lectin RS20L from phytopathogen Ralstonia solanacearum. In \textit{XVIII. setkání biochemiků a molekulárních biologů}. 2017. ISBN~978-80-210-8765-1.
|
