| ZUBAL, L., W. BONANI, D. MANIGLIO, R. CECCATO, D. RENCIUK, Aleš HAMPL, C. MIGLIARESI, J. JANCAR a L. VOJTOVA. Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions. eXPRESS Polymer Letters. Budapest: Budapest University of Technology and Economics, Department of Polymer Engineering, 2018, roč. 12, č. 2, s. 159-170. ISSN 1788-618X. Dostupné z: https://dx.doi.org/10.3144/expresspolymlett.2018.14. |
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@article{1405279,
author = {Zubal, L. and Bonani, W. and Maniglio, D. and Ceccato, R. and Renciuk, D. and Hampl, Aleš and Migliaresi, C. and Jancar, J. and Vojtova, L.},
article_location = {Budapest},
article_number = {2},
doi = {http://dx.doi.org/10.3144/expresspolymlett.2018.14},
keywords = {biocompatible polymers; collagen fibrillogenesis; structural analysis; viscoelastic properties; protein self-assembly},
language = {eng},
issn = {1788-618X},
journal = {eXPRESS Polymer Letters},
title = {Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions},
url = {http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=D1S4CRof7Nzi9hmIBYJ&page=1&doc=1},
volume = {12},
year = {2018}
}
TY - JOUR
ID - 1405279
AU - Zubal, L. - Bonani, W. - Maniglio, D. - Ceccato, R. - Renciuk, D. - Hampl, Aleš - Migliaresi, C. - Jancar, J. - Vojtova, L.
PY - 2018
TI - Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions
JF - eXPRESS Polymer Letters
VL - 12
IS - 2
SP - 159-170
EP - 159-170
PB - Budapest University of Technology and Economics, Department of Polymer Engineering
SN - 1788618X
KW - biocompatible polymers
KW - collagen fibrillogenesis
KW - structural analysis
KW - viscoelastic properties
KW - protein self-assembly
UR - http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=D1S4CRof7Nzi9hmIBYJ&page=1&doc=1
N2 - Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.
ER -
ZUBAL, L., W. BONANI, D. MANIGLIO, R. CECCATO, D. RENCIUK, Aleš HAMPL, C. MIGLIARESI, J. JANCAR a L. VOJTOVA. Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions. \textit{eXPRESS Polymer Letters}. Budapest: Budapest University of Technology and Economics, Department of Polymer Engineering, 2018, roč.~12, č.~2, s.~159-170. ISSN~1788-618X. Dostupné z: https://dx.doi.org/10.3144/expresspolymlett.2018.14.
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