A non-invasive electromechanical system to study cardiac
excitation-contraction coupling

a 2017

A non-invasive electromechanical system to study cardiac excitation-contraction coupling

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Roberto RAITERI et. al.

Základní údaje

Originální název

A non-invasive electromechanical system to study cardiac excitation-contraction coupling

Autoři

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Roberto RAITERI, Vladimír ROTREKL a Petr SKLÁDAL

Vydání

AFM Biomed Conference, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Příznaky

Mezinárodní význam

Změněno: 21. 5. 2018 12:44, MUDr. Martin Pešl, Ph.D.

Anotace

V originále

Abstract Body: The prolonged alterations of the two-stages transducing process known as excitation-contraction coupling (ECC) ultimately leads to cardiac tissue remodeling and heart failure1. The use of a highly-sensitive and non-invasive cell-based biosensor can sharpen significantly the evaluation of the in vitro phenotype and novel cardiac drugs2. We have set up and tested an experimental system based on Atomic Force Microscopy (AFM) and MicroElectrode Array-based ElectroPhysiology (MEA-EP), to monitor the ECC components of human pluripotent stem cell-derived cardiac constructs, in the form of an embryoid body (EB)3. The experimental system was tested in basal condition and under drug trials against cardioactive species. The detected response confirmed an immature phenotype, showed multiple pacing sites in the cardiac constructs, and the modulation of the beating rate and force. On the road to implement a complete ECC monitoring system, the proposed combination boosts the informational content of a single analysis, thus leading to refined evaluation of in vitro models and novel cardiac drugs.Figure. Graphical representation of the combined electromechanical system for ECC monitoring References:1. Lou Q, Fedorov V V., Glukhov A V. et al., Circulation 123, 2011. 2. Pesl M, Pribyl J et al.,J Mol Recognit e2602 2016. 3. Pesl M, Pribyl J et al.,Biosens Bioelectron 85, 2016.

Návaznosti

MUNI/A/1010/2016, interní kód MU

Název: Efekt elektroporační ablace na lidské srdeční buňky

Investor: Masarykova univerzita, Efekt elektroporační ablace na lidské srdeční buňky, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Roberto RAITERI, Vladimír ROTREKL a Petr SKLÁDAL. A non-invasive electromechanical system to study cardiac excitation-contraction coupling. In AFM Biomed Conference. 2017.

@proceedings{1405789,
   author = {Caluori, Guido and Přibyl, Jan and Pešl, Martin and Jelínková, Šárka and Raiteri, Roberto and Rotrekl, Vladimír and Skládal, Petr},
   booktitle = {AFM Biomed Conference},
   language = {eng},
   title = {A non-invasive electromechanical system to study cardiac excitation-contraction coupling},
   year = {2017}
}

TY  - CONF
ID  - 1405789
AU  - Caluori, Guido - Přibyl, Jan - Pešl, Martin - Jelínková, Šárka - Raiteri, Roberto - Rotrekl, Vladimír - Skládal, Petr
PY  - 2017
TI  - A non-invasive electromechanical system to study cardiac excitation-contraction coupling
N2  - Abstract Body: The prolonged alterations of the two-stages transducing process known as excitation-contraction coupling (ECC) ultimately leads to cardiac tissue remodeling and heart failure1. The use of a highly-sensitive and non-invasive cell-based biosensor can sharpen significantly the evaluation of the in vitro phenotype and novel cardiac drugs2. We have set up and tested an experimental system based on Atomic Force Microscopy (AFM) and MicroElectrode Array-based ElectroPhysiology (MEA-EP), to monitor the ECC components of human pluripotent stem cell-derived cardiac constructs, in the form of an embryoid body (EB)3. The experimental system was tested in basal condition and under drug trials against cardioactive species. The detected response confirmed an immature phenotype, showed multiple pacing sites in the cardiac constructs, and the modulation of the beating rate and force. On the road to implement a complete ECC monitoring system, the proposed combination boosts the informational content of a single analysis, thus leading to refined evaluation of in vitro models and novel cardiac drugs.Figure. Graphical representation of the combined electromechanical system for ECC monitoring References:1. Lou Q, Fedorov V V., Glukhov A V. et al., Circulation 123, 2011. 2. Pesl M, Pribyl J et al.,J Mol Recognit e2602 2016. 3. Pesl M, Pribyl J et al.,Biosens Bioelectron 85, 2016.
ER  -

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