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@article{1406031,
author = {Perestrelo, Ana Rubina and Cruz, Jorge Oliver de la and Caluori, Guido and Pagliari, Stefania and Vinarský, Vladimír and Žampachová, Víta and Horváth, Vladimír and Pešl, Martin and Skládal, Petr and Nascimento, Diana S and PintoanddoandÓ, Perpétua and Forte, Giancarlo},
article_number = {Suppl. 2},
language = {eng},
issn = {1473-2262},
journal = {European Cells and Materials},
title = {Contribution of heart extracellular matrix bioscaffolds to disclose the mechanic dynamics behind cardiac pathologies},
url = {http://www.ecmjournal.org/P235},
volume = {33},
year = {2017}
}
TY - JOUR
ID - 1406031
AU - Perestrelo, Ana Rubina - Cruz, Jorge Oliver de la - Caluori, Guido - Pagliari, Stefania - Vinarský, Vladimír - Žampachová, Víta - Horváth, Vladimír - Pešl, Martin - Skládal, Petr - Nascimento, Diana S - Pinto-do-Ó, Perpétua - Forte, Giancarlo
PY - 2017
TI - Contribution of heart extracellular matrix bioscaffolds to disclose the mechanic dynamics behind cardiac pathologies
JF - European Cells and Materials
VL - 33
IS - Suppl. 2
SP - P235
EP - P235
SN - 14732262
UR - http://www.ecmjournal.org/P235
N2 - The complex architecture of cardiac extracellular matrix (ECM) provides a unique 3D environment fundamental for cardiac development, homeostasis, and functionality. The derangement of ECM integrity occurring during the onset and progression of cardiac diseases drives a modification in the nanotopography and mechano-physical properties of the ECM itself, thus impairing cardiac cell contractility and proper organ function. Furthermore, our immunohistochemistry and gene expression analysis, on infarcted mouse hearts and human cardiac biopsies, confirms that the dramatic structural changes triggered by myocardial infarction and tissue remodelling, leading to heart failure, causes aswitch in cardiac cell mechanosome.Cardiac decellularized ECM (dECM) obtained from physiological and pathological specimens feature the three dimensional cues, mechanical properties, chemical complexity and the native organization of heart tissue in healthy and diseased condition. For this reason, they are here proposed as an in vitromodel to investigate the mechanisms of cell-ECM interaction, especially consequent to cardiac pathologies.In this study, we show that second harmonic generation imaging, scanning electron microscopy and atomic force microscopy can be implemented to obtain high-resolution 3D maps of cardiac dECM structure and mechanical properties, as an essential asset to the conventional immunostaining and protein analyses. Furthermore, recellularization of physiological and pathological myocardial scaffolds resulted in distinct cell-dECM interactions, thus bringing further hints to disclose a possible window of action for heart cell therapies. Altogether, our findings show that cardiac dECMs are powerful and reliable toolboxes to monitor cardiac nanostructural changes and to investigate cardiac system mechanobiology.
ER -
PERESTRELO, Ana Rubina, Jorge Oliver de la CRUZ, Guido CALUORI, Stefania PAGLIARI, Vladimír VINARSKÝ, Víta ŽAMPACHOVÁ, Vladimír HORVÁTH, Martin PEŠL, Petr SKLÁDAL, Diana S NASCIMENTO, Perpétua PINTO-DO-Ó a Giancarlo FORTE. Contribution of heart extracellular matrix bioscaffolds to disclose the mechanic dynamics behind cardiac pathologies. \textit{European Cells and Materials}. 2017, roč.~33, Suppl. 2, s.~P235. ISSN~1473-2262.
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