Detection and Quantification of Listeria monocytogenes in
Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined
by Culture Methods and Real-time PCR

J 2017

Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

MORÁVKOVÁ, Monika, Veronika VERBÍKOVÁ, Veronika MICHNÁ, Vladimír BABÁK, Hana CAHLÍKOVÁ et. al.

Základní údaje

Originální název

Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

Autoři

MORÁVKOVÁ, Monika, Veronika VERBÍKOVÁ, Veronika MICHNÁ, Vladimír BABÁK, Hana CAHLÍKOVÁ, Renata KARPÍŠKOVÁ a Petr KRÁLÍK

Vydání

Journal of Food and Nutrition Research, 2017, 2333-1119

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.12691/jfnr-5-11-6

Klíčová slova anglicky

Listeria monocytogenes;Foodborne pathogens;Molecular methods;qPCR;Ready-to-eat

Štítky

Změněno: 31. 3. 2018 13:01, Ing. Nicole Zrilić

Anotace

V originále

In this study, a total of 175 samples of ready-to-eat vegetables, frozen vegetables and sprouted seeds originating in 10 states of the European Union and from 32 manufacturers were collected during a period of one year and examined for the presence of Listeria monocytogenes using standard culture methods and qPCR. In addition to these methods, isolation of Listeria monocytogenes was also carried out following a unified sample preparation for combined downstream use in culture and qPCR analysis. Standard culture and culture preceded by unified sample preparation, showed that L. monocytogenes was present in 6.9% and 11.4% of analyzed samples, respectively, in low numbers. Application of qPCR revealed only 2.3% of samples to be positive for L. monocytogenes in small quantities (less than 10 cells/gram). A statistically significant higher occurrence of L. monocytogenes was seen in frozen vegetables compared to ready to eat vegetables (p<0.01; Fisher’s exact post-hoc tests with Bonferroni’s correction) or sprouts (p<0.05; Fisher’s exact post-hoc tests with Bonferroni’s correction). Therefore, temperature abuse in food containing pieces of frozen vegetables without any processing such as cooking, may pose health risks, especially for sensitive individuals such as pregnant women, children, the elderly, and immunocompromised individuals.

Citovat

MORÁVKOVÁ, Monika, Veronika VERBÍKOVÁ, Veronika MICHNÁ, Vladimír BABÁK, Hana CAHLÍKOVÁ, Renata KARPÍŠKOVÁ a Petr KRÁLÍK. Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR. Journal of Food and Nutrition Research. 2017, roč. 5/2017, č. 11, s. 832-837. ISSN 2333-1119. Dostupné z: https://dx.doi.org/10.12691/jfnr-5-11-6.

@article{1407100,
   author = {Morávková, Monika and Verbíková, Veronika and Michná, Veronika and Babák, Vladimír and Cahlíková, Hana and Karpíšková, Renata and Králík, Petr},
   article_number = {11},
   doi = {http://dx.doi.org/10.12691/jfnr-5-11-6},
   keywords = {Listeria monocytogenes;Foodborne pathogens;Molecular methods;qPCR;Ready-to-eat},
   language = {eng},
   issn = {2333-1119},
   journal = {Journal of Food and Nutrition Research},
   note = {Nepřenášet do RIV! Veronika Michná (Huvarová) je sice studentka PřF MU, ale nemá afiliaci k MU!},
   title = {Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR},
   url = {http://pubs.sciepub.com/jfnr/5/11/6/},
   volume = {5/2017},
   year = {2017}
}

TY  - JOUR
ID  - 1407100
AU  - Morávková, Monika - Verbíková, Veronika - Michná, Veronika - Babák, Vladimír - Cahlíková, Hana - Karpíšková, Renata - Králík, Petr
PY  - 2017
TI  - Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR
JF  - Journal of Food and Nutrition Research
VL  - 5/2017
IS  - 11
SP  - 832-837
EP  - 832-837
SN  - 23331119
N1  - Nepřenášet do RIV! Veronika Michná (Huvarová) je sice studentka PřF MU, ale nemá afiliaci k MU!
KW  - Listeria monocytogenes;Foodborne pathogens;Molecular methods;qPCR;Ready-to-eat
UR  - http://pubs.sciepub.com/jfnr/5/11/6/
N2  - In this study, a total of 175 samples of ready-to-eat vegetables, frozen vegetables and sprouted seeds originating in 10 states of the European Union and from 32 manufacturers were collected during a period of one year and examined for the presence of Listeria monocytogenes using standard culture methods and qPCR. In addition to these methods, isolation of Listeria monocytogenes was also carried out following a unified sample preparation for combined downstream use in culture and qPCR analysis. Standard culture and culture preceded by unified sample preparation, showed that L. monocytogenes was present in 6.9% and 11.4% of analyzed samples, respectively, in low numbers. Application of qPCR revealed only 2.3% of samples to be positive for L. monocytogenes in small quantities (less than 10 cells/gram). A statistically significant higher occurrence of L. monocytogenes was seen in frozen vegetables compared to ready to eat vegetables (p<0.01; Fisher’s exact post-hoc tests with Bonferroni’s correction) or sprouts (p<0.05; Fisher’s exact post-hoc tests with Bonferroni’s correction). Therefore, temperature abuse in food containing pieces of frozen vegetables without any processing such as cooking, may pose health risks, especially for sensitive individuals such as pregnant women, children, the elderly, and immunocompromised individuals.
ER  -

MORÁVKOVÁ, Monika, Veronika VERBÍKOVÁ, Veronika MICHNÁ, Vladimír BABÁK, Hana CAHLÍKOVÁ, Renata KARPÍŠKOVÁ a Petr KRÁLÍK. Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR. \textit{Journal of Food and Nutrition Research}. 2017, roč.~5/2017, č.~11, s.~832-837. ISSN~2333-1119. Dostupné z: https://dx.doi.org/10.12691/jfnr-5-11-6.

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