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@article{1407226, author = {Smrčka, Filip and Lubal, Přemysl and Šídlo, Michal}, article_location = {Wien}, article_number = {11}, doi = {http://dx.doi.org/10.1007/s00706-017-2043-4}, keywords = {Luminescent Eu(III) complex; Biosensor; Carbonate; Urease enzyme; Urea determination}, language = {eng}, issn = {0026-9247}, journal = {Monatshefte fur Chemie}, title = {The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand}, url = {https://link.springer.com/article/10.1007%2Fs00706-017-2043-4}, volume = {148}, year = {2017} }
TY - JOUR ID - 1407226 AU - Smrčka, Filip - Lubal, Přemysl - Šídlo, Michal PY - 2017 TI - The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand JF - Monatshefte fur Chemie VL - 148 IS - 11 SP - 1945-1952 EP - 1945-1952 PB - Springer Wien SN - 00269247 KW - Luminescent Eu(III) complex KW - Biosensor KW - Carbonate KW - Urease enzyme KW - Urea determination UR - https://link.springer.com/article/10.1007%2Fs00706-017-2043-4 L2 - https://link.springer.com/article/10.1007%2Fs00706-017-2043-4 N2 - The Eu(III) ternary complex with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (H(3)DO3A) and picolinate "antenna" ligand ([Eu(DO3A)(L)](-)) reacts with carbonate anions to form the more stable non-fluorescent [Eu(DO3A)(CO3)](2-) complex. This substitution reaction was employed for indirect determination of carbonate formed in course of urea hydrolysis catalyzed by the urease enzyme. This new analytical procedure was proposed for the determination of both analytes (urea, urease) and this sensitive luminescence-based biosensor can be used in both kinetic time-dependent and equilibrium end-point modes. This method, with analytical figures of merit (limit of detection 0.05 mM, dynamic range 0.05-5.00 mM), does not show any systematic error and therefore can be utilized for urea analysis in biological samples (e.g., urine). It was also demonstrated that some metal ions (e.g., Zn(II), Pb(II), Cd(II), Ag(I), etc.) inhibit the enzymatic hydrolysis of urea and this effect can be utilized for their determination; therefore, it is recommended to add EDTA as masking agent prior to enzymatic urea analysis. ER -
SMRČKA, Filip, Přemysl LUBAL a Michal ŠÍDLO. The urea biosensor based on luminescence of Eu(III) ternary complex of DO3A ligand. \textit{Monatshefte fur Chemie}. Wien: Springer Wien, 2017, roč.~148, č.~11, s.~1945-1952. ISSN~0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-017-2043-4.
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