Lactobacillus caviae sp nov., an obligately heterofermentative
bacterium isolated from the oral cavity of a guinea pig (Cavia
aperea f. porcellus)

J 2017

Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)

KILLER, Jiří, Radko PECHAR, Pavel ŠVEC, Hana SALMONOVÁ, Roman ŠVEJSTIL et. al.

Základní údaje

Originální název

Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)

Autoři

KILLER, Jiří (203 Česká republika), Radko PECHAR (203 Česká republika), Pavel ŠVEC (203 Česká republika, garant, domácí), Hana SALMONOVÁ (203 Česká republika), Roman ŠVEJSTIL (203 Česká republika), Martina GEIGEROVÁ (203 Česká republika), Vojtěch RADA (203 Česká republika), Eva VLKOVÁ (203 Česká republika) a Chahrazed MEKADIM (12 Alžírsko)

Vydání

International Journal of Systematic and Evolutionary Microbiology, London, Microbiology Society, 2017, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.932

Kód RIV

RIV/00216224:14310/17:00099979

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/ijsem.0.002044

UT WoS

000409533100064

Klíčová slova anglicky

Lactobacillus sp. nov.; oral cavity; guinea pig; MLSA

Štítky

NZ, rivok

Změněno: 6. 4. 2018 10:58, Ing. Nicole Zrilić

Anotace

V originále

A Gram-stain-positive, facultatively anaerobic, and catalase-and oxidase-negative bacterial strain designated MOZM2(T), having 98.4% 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016(T), was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42% degree of genetic similarity between the novel strain and L. reuteri DSM 20016(T). Strain MOZM2(T) degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2(T) and L. reuteri DSM 20016(T) in their proportions of C-8:0, C-14:1, C-17:0, C-18:2 omega 6t and C-20:0 fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G +C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name Lactobacillus caviae sp. nov. is proposed, of which the type strain is MOZM2(T) (= CCM 8609(T) = DSM 100239(T) = LMG 28780(T)).

Citovat

KILLER, Jiří, Radko PECHAR, Pavel ŠVEC, Hana SALMONOVÁ, Roman ŠVEJSTIL, Martina GEIGEROVÁ, Vojtěch RADA, Eva VLKOVÁ a Chahrazed MEKADIM. Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus). International Journal of Systematic and Evolutionary Microbiology. London: Microbiology Society, 2017, roč. 67, č. 8, s. 2903-2909. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.002044.

@article{1407872,
   author = {Killer, Jiří and Pechar, Radko and Švec, Pavel and Salmonová, Hana and Švejstil, Roman and Geigerová, Martina and Rada, Vojtěch and Vlková, Eva and Mekadim, Chahrazed},
   article_location = {London},
   article_number = {8},
   doi = {http://dx.doi.org/10.1099/ijsem.0.002044},
   keywords = {Lactobacillus sp. nov.; oral cavity; guinea pig; MLSA},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)},
   url = {http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002044},
   volume = {67},
   year = {2017}
}

TY  - JOUR
ID  - 1407872
AU  - Killer, Jiří - Pechar, Radko - Švec, Pavel - Salmonová, Hana - Švejstil, Roman - Geigerová, Martina - Rada, Vojtěch - Vlková, Eva - Mekadim, Chahrazed
PY  - 2017
TI  - Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 67
IS  - 8
SP  - 2903-2909
EP  - 2903-2909
PB  - Microbiology Society
SN  - 14665026
KW  - Lactobacillus sp. nov.
KW  - oral cavity
KW  - guinea pig
KW  - MLSA
UR  - http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002044
L2  - http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002044
N2  - A Gram-stain-positive, facultatively anaerobic, and catalase-and oxidase-negative bacterial strain designated MOZM2(T), having 98.4% 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016(T), was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42% degree of genetic similarity between the novel strain and L. reuteri DSM 20016(T). Strain MOZM2(T) degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2(T) and L. reuteri DSM 20016(T) in their proportions of C-8:0, C-14:1, C-17:0, C-18:2 omega 6t and C-20:0 fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G +C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name Lactobacillus caviae sp. nov. is proposed, of which the type strain is MOZM2(T) (= CCM 8609(T) = DSM 100239(T) = LMG 28780(T)).
ER  -

KILLER, Jiří, Radko PECHAR, Pavel ŠVEC, Hana SALMONOVÁ, Roman ŠVEJSTIL, Martina GEIGEROVÁ, Vojtěch RADA, Eva VLKOVÁ a Chahrazed MEKADIM. Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus). \textit{International Journal of Systematic and Evolutionary Microbiology}. London: Microbiology Society, 2017, roč.~67, č.~8, s.~2903-2909. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.002044.

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