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@article{1408673, author = {Sharma, Anežka and Bányiová, Katarína and Babica, Pavel and El Yamani, Naouale and Collins, Andrew Richard and Čupr, Pavel}, article_location = {AMSTERDAM}, article_number = {September}, doi = {http://dx.doi.org/10.1016/j.scitotenv.2017.03.043}, keywords = {trans/cis-EHMC; Isomerization; Human risk assessment; Genotoxicity; Adult human liver stem cells; High-throughput comet assay}, language = {eng}, issn = {0048-9697}, journal = {Science of the Total Environment}, title = {Different DNA damage response of cis and trans isomers of commonly used UV filter after the exposure on adult human liver stem cells and human lymphoblastoid cells}, url = {https://www.sciencedirect.com/science/article/pii/S0048969717305478?via%3Dihub}, volume = {593}, year = {2017} }
TY - JOUR ID - 1408673 AU - Sharma, Anežka - Bányiová, Katarína - Babica, Pavel - El Yamani, Naouale - Collins, Andrew Richard - Čupr, Pavel PY - 2017 TI - Different DNA damage response of cis and trans isomers of commonly used UV filter after the exposure on adult human liver stem cells and human lymphoblastoid cells JF - Science of the Total Environment VL - 593 IS - September SP - 18-26 EP - 18-26 PB - Elsevier Science BV SN - 00489697 KW - trans/cis-EHMC KW - Isomerization KW - Human risk assessment KW - Genotoxicity KW - Adult human liver stem cells KW - High-throughput comet assay UR - https://www.sciencedirect.com/science/article/pii/S0048969717305478?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0048969717305478?via%3Dihub N2 - 2-ethylhexyl 4-methoxycinnamate (EHMC), used in many categories of personal care products (PCPs), is one of the most discussed ultraviolet filters because of its endocrine-disrupting effects. EHMC is unstable in sunlight and can be transformed from trans-EHMC to emergent cis-EHMC. Toxicological studies are focusing only on transEHMC; thus the toxicological data for cis-EHMC are missing. In this study, the in vitro genotoxic effects of trans- and cis-EHMC on adult human liver stem cells HL1-hT1 and human-derived lymphoblastoid cells TK-6 using a high-throughput comet assay were studied. TK-6 cells treated with cis-EHMC showed a high level of DNA damage when compared to untreated cells in concentrations 1.56 to 25 mu g mL(-1). trans-EHMC showed genotoxicity after exposure to the two highest concentrations 12.5 and 25 mu g mL(-1). The increase in DNA damage on HL1-hT1 cells induced by cis-EHMC and transEHMC was detected at the concentration 25 pg mL-1. The No observed adverse effect level (NOAEL, mg kg lbw day I) was determined using a Quantitative in vitro to in vivo extrapolation (QIVIVE) approach: NOAEL(trans-EHMC) = 3.07, NOAEL(cis-EHMC) = 0.30 for TK-6 and NOAEL(trans-EHMC) = 26.46, NOAEL(cis-EHMC) = 20.36 for HL1-hT1. The hazard index (HI) was evaluated by comparing the reference dose (RID, mg kg(-1) bw day(-1)) obtained from our experimental data with the chronic daily intake (CDI) of the female population. Using comet assay experimental data with the more sensitive TK-6 cells, HIcis-EHMC was 7 times higher than HItrans-EHMC In terms of CDI, relative contributions were; dermal exposure route > oral > inhalation. According to our results we recommend the RfD(trans-EHMC) = 0.20 and RfD(cis-EHMC) = 0.02 for trans-EHMC and cis-EHMC, respectively, to use for human health risk assessment. The significant difference in trans-EHMC and cis-EHMC response points to the need for toxicological reevaluation and application reassessment of both isomers in PCPs. ER -
SHARMA, Anežka, Katarína BÁNYIOVÁ, Pavel BABICA, Naouale EL YAMANI, Andrew Richard COLLINS a Pavel ČUPR. Different DNA damage response of cis and trans isomers of commonly used UV filter after the exposure on adult human liver stem cells and human lymphoblastoid cells. \textit{Science of the Total Environment}. AMSTERDAM: Elsevier Science BV, 2017, roč.~593, September, s.~18-26. ISSN~0048-9697. Dostupné z: https://dx.doi.org/10.1016/j.scitotenv.2017.03.043.
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