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@inproceedings{1409298, author = {Castilla, Carlos and Maška, Martin and Sorokin, Dmitry and Meijering, Erik and OrtizanddeandSolorzano, Carlos}, address = {Washington}, booktitle = {15th IEEE International Symposium on Biomedical Imaging}, doi = {http://dx.doi.org/10.1109/ISBI.2018.8363605}, keywords = {Cell segmentation; Convolutional Neural Networks; Chan-Vese model; Filopodia}, howpublished = {elektronická verze "online"}, language = {eng}, location = {Washington}, isbn = {978-1-5386-3636-7}, pages = {413-417}, publisher = {IEEE}, title = {Segmentation of Actin-Stained 3D Fluorescent Cells with Filopodial Protrusions using Convolutional Neural Networks}, url = {https://doi.org/10.1109/ISBI.2018.8363605}, year = {2018} }
TY - JOUR ID - 1409298 AU - Castilla, Carlos - Maška, Martin - Sorokin, Dmitry - Meijering, Erik - Ortiz-de-Solorzano, Carlos PY - 2018 TI - Segmentation of Actin-Stained 3D Fluorescent Cells with Filopodial Protrusions using Convolutional Neural Networks PB - IEEE CY - Washington SN - 9781538636367 KW - Cell segmentation KW - Convolutional Neural Networks KW - Chan-Vese model KW - Filopodia UR - https://doi.org/10.1109/ISBI.2018.8363605 N2 - We present the architecture, training strategy and evaluation of a convolutional neural network (CNN) designed for the segmentation of actin-stained cells in 3D+t confocal microscopy image data. The segmentation performance of the CNN is evaluated using time-lapse sequences of lung adenocarcinoma cells with three genetically distinct variants of the tubulin adaptor protein, a key protein in the process of assembly of the cell cytoskeleton, displaying three different phenotypes in regards to the morphology of the cells and in particular, to the number and length of filopodial structures. We show that the CNN significantly outperforms a baseline method based on the minimization of the Chan-Vese model using graph cuts, and we discuss the inherent benefits of using the CNN over the baseline method. ER -
CASTILLA, Carlos, Martin MAŠKA, Dmitry SOROKIN, Erik MEIJERING a Carlos ORTIZ-DE-SOLORZANO. Segmentation of Actin-Stained 3D Fluorescent Cells with Filopodial Protrusions using Convolutional Neural Networks. Online. In \textit{15th IEEE International Symposium on Biomedical Imaging}. Washington: IEEE, 2018, s.~413-417. ISBN~978-1-5386-3636-7. Dostupné z: https://dx.doi.org/10.1109/ISBI.2018.8363605.
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