Click chemistry-based tracking reveals putative cell
wall-located auxin binding sites in expanding cells

J 2017

Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells

MRAVEC, J., S.K. KRACUN, Elena ZEMLYANSKAYA, M.G. RYDAHL, X.Y. GUO et. al.

Základní údaje

Originální název

Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells

Autoři

MRAVEC, J. (208 Dánsko), S.K. KRACUN (208 Dánsko), Elena ZEMLYANSKAYA (112 Bělorusko, domácí), M.G. RYDAHL (208 Dánsko), X.Y. GUO (208 Dánsko), M. PICMANOVA (208 Dánsko), K.K. SORENSEN (208 Dánsko), Kamil RŮŽIČKA (203 Česká republika, garant, domácí) a W.G.T. WILLATS (826 Velká Británie a Severní Irsko)

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2017, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.122

Kód RIV

RIV/00216224:14740/17:00095528

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41598-017-16281-w

UT WoS

000416118300012

Klíčová slova anglicky

MEMBRANE H+-ATPASE; BIOLOGICAL-ACTIVITY; AZIDO AUXINS; ARABIDOPSIS; EXPANSION; PROTEINS; ELONGATION; INHIBITION; TRANSPORT; GROWTH

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2018 16:51, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation.

Návaznosti

GA16-26428S, projekt VaV

Název: Alternativní sestřih jako regulátor auxinového transportu

Investor: Grantová agentura ČR, Alternativní sestřih jako regulátor auxinového transportu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MRAVEC, J., S.K. KRACUN, Elena ZEMLYANSKAYA, M.G. RYDAHL, X.Y. GUO, M. PICMANOVA, K.K. SORENSEN, Kamil RŮŽIČKA a W.G.T. WILLATS. Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2017, roč. 7, NOV, s. 15988-16000. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-017-16281-w.

@article{1409441,
   author = {Mravec, J. and Kracun, S.K. and Zemlyanskaya, Elena and Rydahl, M.G. and Guo, X.Y. and Picmanova, M. and Sorensen, K.K. and Růžička, Kamil and Willats, W.G.T.},
   article_location = {LONDON},
   article_number = {NOV},
   doi = {http://dx.doi.org/10.1038/s41598-017-16281-w},
   keywords = {MEMBRANE H+-ATPASE; BIOLOGICAL-ACTIVITY; AZIDO AUXINS; ARABIDOPSIS; EXPANSION; PROTEINS; ELONGATION; INHIBITION; TRANSPORT; GROWTH},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells},
   url = {https://www.nature.com/articles/s41598-017-16281-w.pdf},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1409441
AU  - Mravec, J. - Kracun, S.K. - Zemlyanskaya, Elena - Rydahl, M.G. - Guo, X.Y. - Picmanova, M. - Sorensen, K.K. - Růžička, Kamil - Willats, W.G.T.
PY  - 2017
TI  - Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells
JF  - Scientific reports
VL  - 7
IS  - NOV
SP  - 15988
EP  - 15988
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - MEMBRANE H+-ATPASE
KW  - BIOLOGICAL-ACTIVITY
KW  - AZIDO AUXINS
KW  - ARABIDOPSIS
KW  - EXPANSION
KW  - PROTEINS
KW  - ELONGATION
KW  - INHIBITION
KW  - TRANSPORT
KW  - GROWTH
UR  - https://www.nature.com/articles/s41598-017-16281-w.pdf
L2  - https://www.nature.com/articles/s41598-017-16281-w.pdf
N2  - Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation.
ER  -

MRAVEC, J., S.K. KRACUN, Elena ZEMLYANSKAYA, M.G. RYDAHL, X.Y. GUO, M. PICMANOVA, K.K. SORENSEN, Kamil RŮŽIČKA a W.G.T. WILLATS. Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2017, roč.~7, NOV, s.~15988-16000. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-017-16281-w.

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