Reprogramming of peripheral blood cells into hiPSCs as a source
of endothelial cells

a 2018

Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ et. al.

Základní údaje

Originální název

Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells

Autoři

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA a Irena KRONTORÁD KOUTNÁ

Vydání

SY-Stem symposium 2018, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

ABSTRACT BOOKLET 2018

Organizační jednotka

Fakulta informatiky

Klíčová slova česky

buněčná reprogramace, endotheliální diferenciace

Klíčová slova anglicky

cellular reprogramming, endothelial differentiation

Změněno: 2. 3. 2018 10:41, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

Recent advances in human induced pluripotent stem cell (hiPSC) research have opened avenues to generate unlimited numbers of endothelial cells (ECs) from easily accessible cell sources, such as the peripheral blood. We reprogrammed peripheral blood mononuclear cells (PBMCs), human umbilical vein endothelial cells (HUVECs) and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. hiPSC-derived ECs were compared to HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts both phenotypically and functionally. An increased number of DNA double-strand breaks (γH2AX) upon reprogramming was observed. However, differentiation into ECs restored a normal number of γH2AX foci. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ŠIMARA, Pavel, Lenka TESAŘOVÁ, Daniela ŘEHÁKOVÁ, Šimon FARKAŠ, Barbara ŠALINGOVÁ, Kateřina KUTÁLKOVÁ, Eva VAVREČKOVÁ, Pavel MATULA, Petr MATULA a Irena KRONTORÁD KOUTNÁ. Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells. In SY-Stem symposium 2018. 2018.

@proceedings{1409570,
   author = {Šimara, Pavel and Tesařová, Lenka and Řeháková, Daniela and Farkaš, Šimon and Šalingová, Barbara and Kutálková, Kateřina and Vavrečková, Eva and Matula, Pavel and Matula, Petr and Krontorád Koutná, Irena},
   booktitle = {SY-Stem symposium 2018},
   keywords = {cellular reprogramming, endothelial differentiation},
   language = {eng},
   title = {Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells},
   url = {https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/},
   year = {2018}
}

TY  - CONF
ID  - 1409570
AU  - Šimara, Pavel - Tesařová, Lenka - Řeháková, Daniela - Farkaš, Šimon - Šalingová, Barbara - Kutálková, Kateřina - Vavrečková, Eva - Matula, Pavel - Matula, Petr - Krontorád Koutná, Irena
PY  - 2018
TI  - Reprogramming of peripheral blood cells into hiPSCs as a source of endothelial cells
KW  - cellular reprogramming, endothelial differentiation
UR  - https://www.imba.oeaw.ac.at/seminars-events/symposia-conferences/sy-stem/programme/abstract-booklet/
N2  - Recent advances in human induced pluripotent stem cell (hiPSC) research have opened avenues to generate unlimited numbers of endothelial cells (ECs) from easily accessible cell sources, such as the peripheral blood. We reprogrammed peripheral blood mononuclear cells (PBMCs), human umbilical vein endothelial cells (HUVECs) and human saphenous vein endothelial cells (HSVECs) into hiPSCs and differentiated them into ECs. hiPSC-derived ECs were compared to HUVECs and HSVECs. hiPSC-derived ECs resembled their natural EC counterparts both phenotypically and functionally. An increased number of DNA double-strand breaks (γH2AX) upon reprogramming was observed. However, differentiation into ECs restored a normal number of γH2AX foci. Peripheral blood from adult donors is a suitable source for the unlimited production of patient-specific ECs through the hiPSC interstage.
ER  -

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