MAMP (microbe-associated molecular pattern)-induced changes in
plasma membrane-associated proteins

J 2017

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

UHLÍKOVÁ, Hana; Martin SOLANSKÝ; Vendula HRDINOVÁ; Ondrej ŠEDO; Tomáš KAŠPAROVSKÝ et. al.

Základní údaje

Originální název

MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins

Autoři

UHLÍKOVÁ, Hana (203 Česká republika, domácí); Martin SOLANSKÝ (203 Česká republika, domácí); Vendula HRDINOVÁ (203 Česká republika, domácí); Ondrej ŠEDO (203 Česká republika, domácí); Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí); Jan HEJÁTKO (203 Česká republika, domácí) a Jan LOCHMAN (203 Česká republika, garant, domácí)

Vydání

Journal of Plant Physiology, JENA, ELSEVIER GMBH, 2017, 0176-1617

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.833

Kód RIV

RIV/00216224:14310/17:00095532

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.jplph.2016.12.005

UT WoS

000397471200006

EID Scopus

2-s2.0-85007570670

Klíčová slova anglicky

Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase

Štítky

CF PROT, NZ

Změněno: 31. 5. 2022 17:06, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.

Návaznosti

GAP501/12/0590, projekt VaV

Název: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Grantová agentura ČR, Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

UHLÍKOVÁ, Hana; Martin SOLANSKÝ; Vendula HRDINOVÁ; Ondrej ŠEDO; Tomáš KAŠPAROVSKÝ; Jan HEJÁTKO a Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. Journal of Plant Physiology. JENA: ELSEVIER GMBH, 2017, roč. 210, March, s. 51-57. ISSN 0176-1617. Dostupné z: https://doi.org/10.1016/j.jplph.2016.12.005.

@article{1409620,
   author = {Uhlíková, Hana and Solanský, Martin and Hrdinová, Vendula and Šedo, Ondrej and Kašparovský, Tomáš and Hejátko, Jan and Lochman, Jan},
   article_location = {JENA},
   article_number = {March},
   doi = {https://doi.org/10.1016/j.jplph.2016.12.005},
   keywords = {Cryptogein; Rab8; Resistance; ATPase; NADP-malic enzyme; Glutamine synthetase},
   language = {eng},
   issn = {0176-1617},
   journal = {Journal of Plant Physiology},
   note = {Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.},
   title = {MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins},
   url = {http://dx.doi.org/10.1016/j.jplph.2016.12.005},
   volume = {210},
   year = {2017}
}

TY  - JOUR
ID  - 1409620
AU  - Uhlíková, Hana - Solanský, Martin - Hrdinová, Vendula - Šedo, Ondrej - Kašparovský, Tomáš - Hejátko, Jan - Lochman, Jan
PY  - 2017
TI  - MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins
JF  - Journal of Plant Physiology
VL  - 210
IS  - March
SP  - 51-57
EP  - 51-57
PB  - ELSEVIER GMBH
SN  - 01761617
N1  - Nejde o článek v odborném periodioku. WoS. Document Type:Editorial Material. Nutné odvést do RIV jko výsledek typu "O" - ostatní.
KW  - Cryptogein
KW  - Rab8
KW  - Resistance
KW  - ATPase
KW  - NADP-malic enzyme
KW  - Glutamine synthetase
UR  - http://dx.doi.org/10.1016/j.jplph.2016.12.005
N2  - Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins. (C) 2016 Elsevier GmbH. All rights reserved.
ER  -

UHLÍKOVÁ, Hana; Martin SOLANSKÝ; Vendula HRDINOVÁ; Ondrej ŠEDO; Tomáš KAŠPAROVSKÝ; Jan HEJÁTKO a Jan LOCHMAN. MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins. \textit{Journal of Plant Physiology}. JENA: ELSEVIER GMBH, 2017, roč.~210, March, s.~51-57. ISSN~0176-1617. Dostupné z: https://doi.org/10.1016/j.jplph.2016.12.005.

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